Overview

  • Product name

    Human IL-23 Matched Antibody Pair Kit
    See all IL-23 kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Range

    15.62 pg/ml - 1000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human IL-23.


    Matched antibody pair kits and reagents deliver consistent, specific, and sensitive results.



    • Batch-to-batch consistency: only recombinant monoclonal antibodies are used in our matched antibody pairs.

    • Specificity: antibody pairs are screened in plasma and serum to ensure specificity in complex samples.

    • Sensitivity: benchmarked against commercially available antibody pairs to ensure equivalent or superior performance compared with the competition.


    Additional buffers and plates are required for the assay. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab221837) which uses the same antibody pair.


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 2 x 96 tests
    Human IL-23 Capture Antibody 1 x 100µg 1 x 20µg
    Human IL-23 Detector Antibody 1 x 25µg 1 x 5µg
    Human IL-23 Lyophilized Protein 1 vial 1 vial
  • Research areas

  • Function

    Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • Tissue specificity

    Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level).
  • Sequence similarities

    Belongs to the IL-6 superfamily.
  • Developmental stage

    Expressed by newborns dendritic cells.
  • Cellular localization

    Secreted. Secreted upon association with IL12B.
  • Information by UniProt
  • Alternative names

    • IL 23
    • IL 23 A
    • IL 23 subunit alpha
    • IL 23A
    • IL 23p19
    • IL-23 subunit alpha
    • IL-23-A
    • IL-23p19
    • IL12B
    • IL23
    • Il23a
    • IL23A_HUMAN
    • IL23P19
    • interleukin 12B
    • Interleukin 23 alpha subunit p19
    • Interleukin 23 p19 subunit
    • interleukin 23 subunit alpha
    • interleukin 23 subunit p19
    • interleukin six, G CSF related factor
    • Interleukin-23 subunit alpha
    • Interleukin-23 subunit p19
    • JKA3 induced upon T cell activation
    • MGC79388
    • P19
    • SGRF
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab222278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • Standard calibration curve. Background subtracted values are graphed.

Protocols

References

ab222278 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab222278.
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