Overview

  • Product name

    Human IL-28A ELISA Kit
    See all IL-28A kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    HUVEC 8 2.8%
  • Sample type

    Cell culture supernatant
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    25.24 pg/ml
  • Range

    98.44 pg/ml - 6300 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 101 % - %

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    IL-28A in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-28A protein in human cell culture supernatant samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    IL-28A, also known as interferon lambda-2, is a cytokine that plays a key role in endothelial antiviral defense signaling. In conjunction with its receptors, IL-28A activates the JAK/STAT signaling pathway leading to the induction of host-defense gene expression. IL-28A is an outcome marker for hepatitis C therapeutic intervention and is actively being researched as an immunoadjuvant.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human IL-28A Capture Antibody 1 x 600µl
    10X Human IL-28A Detector Antibody 1 x 600µl
    Human IL-28A Lyophilized Recombinant Protein 2 vials
    Antibody Diluent CPI 1 x 6ml
    10X Wash Buffer PT (ab206977) 1 x 20ml
    TMB Development Solution 1 x 12ml
    Sample Diluent NS (ab193972) 1 x 50ml
    Stop Solution 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Plate Seals 1 unit
  • Research areas

  • Function

    Cytokine with immunomodulatory activity. Up-regulates MHC class I antigen expression. Displays potent antiviral activity. Also displays antitumor activity. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway.
  • Sequence similarities

    Belongs to the IL-28/IL-29 family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Cytokine ZCYTO20
    • IFN-lambda-2
    • IFNL2
    • IL-28A
    • IL28A
    • IL28A_HUMAN
    • Interferon lambda 2
    • Interferon lambda-2
    • Interleukin 28A
    • interleukin 28A (interferon, lambda 2)
    • Interleukin-28A
    • ZCYTO20
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab238269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • HUVEC cells were stimulated by adding human IFN-a at 100 IU/mL for 20 hours, followed by the addition of Poly(I:C) at 30 µg/mL for 3 hours. The concentrations of IL28A were measured in duplicates, interpolated from the IL28A standard curves and corrected for sample dilution. Undiluted samples are as follows: unstimulated HUVEC cell supernatant 100% and stimulated HUVEC cell supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). IL28A was not detectable in unstimulated HUVEC cell culture supernatant. The mean IL28A concentration was determined to be 545.5 pg/mL in stimulated HUVEC cell culture supernatant.

Protocols

References

ab238269 has not yet been referenced specifically in any publications.

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