Human IL-28A ELISA Kit (Interleukin-28A) (ab100567)

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Overview

  • Product name
    Human IL-28A ELISA Kit (Interleukin-28A)
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 15 pg/ml
  • Range
    13.72 pg/ml - 10000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 105.2 95% - 114%
    Plasma 110.4 84% - 120%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-28A (Interleukin 28A) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-28A in plasma and cell culture supernatants.


    This assay employs an antibody specific for Human IL-28A coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-28A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-28A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-28A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect endogenous Human IL-28A in normal serum with ab100567, only in serum spiked with Human IL-28A.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Typical standard curve
    Typical standard curve

    Representative standard curve using ab100567

Protocols

References

ab100567 has not yet been referenced specifically in any publications.

Publishing research using ab100567? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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4 reviews or Q&A

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Has cross reactivity against human IL28B and IL29 been assessed?

Unfortunately, we do not have any cross reactivity data with ab100567 and IL-28B. Since human IL-28A does have very high homology with human IL-28B (96%) it is possible that the antibody pair in the kit is also detecting IL-28B. However, without data t...

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Ihave a Customer, who bought an ELISA kit for detection of IL28A (cat. ab100567). He needs to know if also the antibody anti-IL28A can detect IL28B isoform. I was searching in the datasheet, but I considered that information is unclear about that.I wil...

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Thank you for your patience.

Unfortunately, it looks like that neither the cross-reactivity has been determined, nor the epitope for this antibody mapped. However, as the two protein are up to 95% identical, it is very likely that the antibody...

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Ihave a Customer, who bought an ELISA kit for detection of IL28A (cat. ab100567). He needs to know if also the antibody anti-IL28A can detect IL28B isoform. I was searching in the datasheet, but I considered that information is unclear about that.I wil...

Read More

Thank you for your inquiry.

Unfortunately, epitope mapping has not been performed for the antibodies used in the ab100567, nor has IL-28B been tested with this kit so the cross reactivity with IL-28B is not known. Therefore this information w...

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I wanted to ask if the human IL28A ELISA kit (cat. ab100567) has cross-react with IL28B, since both molecules have a high homology (96%) and I need to know if you have done studies that show that levels of IL28B not interfere with the specific measurem...

Read More

Thank you for contacting us.

Unfortunately, we do not have any cross reactivity data of ab100567 with IL-28B. Since human IL-28A does have very high homology with human IL-28B (96%), it is possible that the antibody pair in the kit will also...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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