Key features and details
- Sensitivity: 20 pg/ml
- Range: 13.72 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman IL-29 ELISA Kit
See all IL-29 kits
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 20 pg/ml
Range13.72 pg/ml - 10000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 124 104% - 143% Serum 97.78 87% - 105% Plasma 112.7 95% - 145%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s IL-29 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-29 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human IL-29 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-29 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-29 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-29 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Human IL-29 2 vials IL-29 Microplate (12 x 8 wells) 1 unit Recombinant Human IL-29 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
FunctionCytokine with immunomodulatory activity. May play a role in antiviral immunity. Up-regulates MHC class I antigen expression. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway.
Sequence similaritiesBelongs to the IL-28/IL-29 family.
- Information by UniProt
- Cytokine ZCYTO21
- IFN lambda 1
ab100568 has not yet been referenced specifically in any publications.