Overview

  • Product name

    Human IL-29 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 2.8%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 2.5%
  • Sample type

    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    7.8 pg/ml
  • Range

    31.3 pg/ml - 3200 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 99 95% - 102%
    Serum 111 107% - 115%
    Cell culture media 106 102% - 109%
    Heparin Plasma 117 116% - 117%
    EDTA Plasma 93 85% - 102%
    Citrate Plasma 88 85% - 91%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    IL-29 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-29 protein in human serum, plasma, and cell culture supernatants.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    IL-29 (interleukin-29), also known as Interferon lambda-1, is a cytokine with antiviral, antitumor and immunomodulatory activities. It plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. IL-29 is a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes, which mediate the antiviral state. IL-29 has a restricted receptor distribution and therefore restricted targets, it is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. IL-29 exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.


    Sensitivity:


    Samples in Sample Diluent 10BP: 7.8 pg/mL.


    Samples in Sample Diluent 50BP: 9.8 pg/mL.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human IL-29 Capture Antibody 1 x 600µl
    10X Human IL-29 Detector Antibody 1 x 600µl
    Human IL-29 Lyophilized Recombinant Protein 2 vials
    Antibody Diluent 4BI 1 x 6ml
    10X Wash Buffer PT (ab206977) 1 x 20ml
    TMB Development Solution 1 x 12ml
    Stop Solution 1 x 12ml
    Sample Diluent NS (ab193972) 1 x 50ml
    Sample Diluent 50BP 1 x 20ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Plate Seals 1 unit
  • Research areas

  • Function

    Cytokine with immunomodulatory activity. May play a role in antiviral immunity. Up-regulates MHC class I antigen expression. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway.
  • Sequence similarities

    Belongs to the IL-28/IL-29 family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Cytokine ZCYTO21
    • IFN lambda 1
    • IFN-lambda-1
    • IFNL1
    • IL-29
    • IL29
    • IL29_HUMAN
    • Interferon lambda 1
    • Interferon lambda-1
    • Interleukin 29 (interferon, lambda 1)
    • Interleukin-29
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab236715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of IL-29 were measured in duplicates, interpolated from the IL-29 standard curves and corrected for sample dilution. Undiluted samples are as follows: 50% HUVEC supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-29 concentration was determined to be 412.4 pg/mL in neat stimulated HUVEC cell culture supernatant.

  • HUVEC cells were cultured for 20 hours in the presence or absence of 100 IU/mL human IFN alpha and 30 µg/mL Poly(I:C). The concentrations of IL-29 were measured in three different dilutions of the supernatant samples in duplicates and interpolated from the IL-29 standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean IL-29 concentration was determined to be 412.4 pg/mL in stimulated HUVEC cell culture supernatant, and undetectable in unstimulated HUVEC cell culture supernatant and RPMI1640 media containing 10% FBS (not shown).

Protocols

References

ab236715 has not yet been referenced specifically in any publications.

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