Human IL-3 ELISA Kit (ab100569)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 16.46 pg/ml - 12000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-3 ELISA Kit
See all IL-3 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 20 pg/ml -
Range
16.46 pg/ml - 12000 pg/ml -
Recovery
100 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 118 110% - 127% Serum 114.4 103% - 122% Plasma 98.82 86% - 118% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s IL-3 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-3 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human IL-3 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimization may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human IL-3 2 vials IL-3 Microplate (12 x 8 wells) 1 unit Recombinant Human IL-3 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes. -
Tissue specificity
Activated T-cells, mast cells, natural killer cells. -
Sequence similarities
Belongs to the IL-3 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Colony stimulating factor multiple
- Hematopoietic growth factor
- IL 3
see all -
Database links
- Entrez Gene: 3562 Human
- Omim: 147740 Human
- SwissProt: P08700 Human
- Unigene: 694 Human
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab100569 has not yet been referenced specifically in any publications.