Key features and details
- Sensitivity: 18.3 pg/ml
- Range: 31.25 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Other biological fluids, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman IL-31 ELISA Kit
See all IL-31 kits
Intra-assay Sample n Mean SD CV% Various 2.5% Inter-assay Sample n Mean SD CV% Various 5.2%
Sample typeCell culture supernatant, Serum, Other biological fluids, Hep Plasma, EDTA Plasma, Cit plasma
Assay typeSandwich (quantitative)
Sensitivity< 18.3 pg/ml
Range31.25 pg/ml - 1000 pg/ml
Sample specific recovery Sample type Average % Range Serum 98 82% - 120%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Human IL-31 ELISA kit (ab273163) for the in vitro qualitative and quantitative determination of IL-31 in supernatants, buffered solutions or serum and plasma samples and other body fluids. This assay will recognise both natural and recombinant human IL-31.
A capture Antibody highly specific for IL-31 has been coated to the wells of the microtitre strip plate provided during manufacture. Binding of IL-31 in samples and known standards to the capture antibodies is completed and then any excess unbound analyte is removed.
During the next incubation period the binding of the biotinylated anti-IL-31 secondary antibody to the analyte occurs. Any excess unbound secondary antibody is then removed.
The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing.
A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of IL-31 present in the samples and standards.
The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of IL-31 in any sample tested.
PlatformMicroplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated Antibody diluent 1 x 7ml Biotinylated Antibody IL-31 1 x 400µl HRP Diluent 1 x 23ml IL-31 Coated Microwell strips 1 unit IL-31 Standard 2 vials Plastic Plate Cover 2 units Standard diluent Buffer 1 x 25ml Stop Reagent 1 x 11ml Streptavidin-HRP 2 x 5µl TMB Substrate 1 x 11ml Wash Buffer 1 x 10ml
FunctionActivates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity.
Tissue specificityDetected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea.
- Information by UniProt
- IL 31
ab273163 has not yet been referenced specifically in any publications.