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    human-il-36-gamma-elisa-il-1f9-ab267633.pdf

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Immunology Innate Immunity Cytokines Interleukins
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Human IL-36 gamma ELISA (IL-1F9) (ab267633)

  • Datasheet
  • SDS
  • Protocol Booklet
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Example data

    Key features and details

    • Sensitivity: 13 pg/ml
    • Range: 12.29 pg/ml - 3000 pg/ml
    • Sample type: Cell culture supernatant, Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

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    Overview

    • Product name

      Human IL-36 gamma ELISA (IL-1F9)
      See all IL36 gamma/IL-1F9 kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall < 10%
      Inter-assay
      Sample n Mean SD CV%
      Overall < 12%
    • Sample type

      Cell culture supernatant, Serum, Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      13 pg/ml
    • Range

      12.29 pg/ml - 3000 pg/ml
    • Recovery

      Sample specific recovery
      Sample type Average % Range
      Serum 120.3 110% - 147%
      Plasma 108.4 100% - 114%
      Cell culture media 107.1 99% - 116%
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      Human IL-36 gamma ELISA Kit is designed for the quantitative determination of IL-36 gamma in cell culture supernatants, plasma and serum samples.


      This assay employs an antibody specific for human IL-36 gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-36 gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-36 gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-36 gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


      This ELISA kit shows no cross-reactivity with the following cytokines tested: Human IL-1F1 (IL-1 alpha), IL-1F2 (IL-1 beta), IL-1F3 (IL-1ra), IL-1F4 (IL-18), IL-1F5 (IL-36ra), IL-1F6 (IL-36 alpha), IL-1F7 (IL-37), IL-1F8 (IL-36 beta), IL-1F10 (IL-38), IL-1F11 (IL-33).

    • Platform

      Pre-coated microplate (12 x 8 well strips)

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 1 x 96 tests
      20X Wash Buffer 1 x 25ml
      250X HRP-Streptavidin Concentrate 1 x 200µl
      5X Assay Diluent 1 x 15ml
      Anti-Human IL-36 gamma coated Microplate (12 x 8 wells) 1 unit
      Biotinylated Anti-Human IL-36 gamma Detection Antibody 2 vials
      Human IL-36 gamma Standard (Lyophilized) 2 vials
      Stop Solution 1 x 8ml
      TMB Substrate Solution 1 x 12ml
    • Research areas

      • Immunology
      • Innate Immunity
      • Cytokines
      • Interleukins
    • Function

      Function as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2. Could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1), that is present in epithelial barriers and takes part in local inflammatory response.
    • Tissue specificity

      Highly expressed in tissues containing epithelial cells: skin, lung, stomach and esophagus. In skin is expressed only in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Up-regulated in lesional psoriasis skin.
    • Sequence similarities

      Belongs to the IL-1 family.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession Q9NZH8 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • IL 1 epsilon
      • IL 1 related protein 2
      • IL 1(EPSILON)
      • IL 1F9
      • IL 1H1
      • IL 1RP2
      • IL-1 epsilon
      • IL-1-related protein 2
      • IL-1F9
      • IL-1H1
      • IL-1RP2
      • IL1E
      • Il1f9
      • IL1F9_HUMAN
      • IL1H1
      • IL1RP2
      • IL36 gamma
      • IL36G
      • Interleukin 1 epsilon
      • Interleukin 1 family member 9
      • Interleukin 1 homolog 1
      • Interleukin 1 related protein 2
      • Interleukin 36 gamma
      • Interleukin-1 epsilon
      • Interleukin-1 family member 9
      • Interleukin-1 homolog 1
      see all
    • Database links

      • Entrez Gene: 56300 Human
      • Omim: 605542 Human
      • SwissProt: Q9NZH8 Human
      • Unigene: 211238 Human

      Images

      • Example data
        Example data

        This standard curve is for demonstration only. A standard curve must be run with each assay.

         

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab267633? Please let us know so that we can cite the reference in this datasheet.

      ab267633 has not yet been referenced specifically in any publications.

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