Human IL-36 gamma ELISA (IL-1F9) (ab267633)
Overview
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Product name
Human IL-36 gamma ELISA (IL-1F9) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
13 pg/ml -
Range
12.29 pg/ml - 3000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 120.3 110% - 147% Plasma 108.4 100% - 114% Cell culture media 107.1 99% - 116% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-36 gamma ELISA Kit is designed for the quantitative determination of IL-36 gamma in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human IL-36 gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-36 gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-36 gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-36 gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: Human IL-1F1 (IL-1 alpha), IL-1F2 (IL-1 beta), IL-1F3 (IL-1ra), IL-1F4 (IL-18), IL-1F5 (IL-36ra), IL-1F6 (IL-36 alpha), IL-1F7 (IL-37), IL-1F8 (IL-36 beta), IL-1F10 (IL-38), IL-1F11 (IL-33).
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 250X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Human IL-36 gamma coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human IL-36 gamma Detection Antibody 2 vials Human IL-36 gamma Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Function as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2. Could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1), that is present in epithelial barriers and takes part in local inflammatory response. -
Tissue specificity
Highly expressed in tissues containing epithelial cells: skin, lung, stomach and esophagus. In skin is expressed only in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Up-regulated in lesional psoriasis skin. -
Sequence similarities
Belongs to the IL-1 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- IL 1 epsilon
- IL 1 related protein 2
- IL 1(EPSILON)
see all -
Database links
- Entrez Gene: 56300 Human
- Omim: 605542 Human
- SwissProt: Q9NZH8 Human
- Unigene: 211238 Human
Images
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Example dataThis standard curve is for demonstration only. A standard curve must be run with each assay.
References
ab267633 has not yet been referenced specifically in any publications.
