Overview

  • Product name

    Human IL-37 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 178pg/ml 7.3 4.1%
    2 16 304pg/ml 11.86 3.9%
    3 16 569pg/ml 30.2 5.3%
    Inter-assay
    Sample n Mean SD CV%
    1 24 216pg/ml 12.3 5.7%
    2 24 365pg/ml 15.33 4.2%
    3 24 610pg/ml 39.04 6.4%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    15.6 pg/ml - 1000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human IL-37 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213798) is designed for the quantitative measurement of Human IL-37 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for IL-37 has been pre-coated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: V46-D218) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-37 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-37 amount of sample captured in plate.

  • Notes

    Interleukin 37 also named IL1F7, is a protein that in humans is encoded by the IL1F7 gene. The protein encoded by this gene is a member of the interleukin 1 cytokine family, and it is mapped to 2q13. In vitro expression analysis showed that IL-37 was a secretory protein. IL-37 could play an important role in the link between innate and adaptive immunity and may be useful for tumor immunotherapy. Meanwhile, IL-37 inhibits the production of inflammatory cytokines in peripheral blood mononuclear cells of patients with systemic lupus erythematosus. In addition, IL-37 emerged as a dual-function cytokine with intra- and extracellular properties for suppressing innate inflammation. Furthermore, IL-37 is a new anti-inflammatory cytokine that plays an important role in protecting against tissue injury during infections via limiting immune and inflammatory reactions.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human IL-37 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- human IL-37 antibody 1 x 130µl
    Lyophilized recombinant human IL-37 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Suppressor of innate inflammatory and immune responses involved in curbing excessive inflammation. This function requires SMAD3. Suppresses, or reduces, proinflammatory cytokine production, including IL1A and IL6, as well as CCL12, CSF1, CSF2, CXCL13, IL1B, IL23A and IL1RN, but spares anti-inflammatory cytokines. Inhibits dendritic cell activation.
  • Tissue specificity

    In general, low constitutive expression, if any, in healthy tissues; high expression in inflammatory counterparts, including in synovial tissues from individuals with active rheumatoid arthritis. Isoform A, isoform B and isoform C are expressed in testis, colon, placenta, lung and lymph node. Isoform D and isoform E were found only in testis and bone marrow. Whereas only isoform A is found in brain, only isoform B in kidney and only isoform C in heart.
  • Sequence similarities

    Belongs to the IL-1 family.
  • Post-translational
    modifications

    Proteolytically converted to the mature form by CASP1.
  • Cellular localization

    Cytoplasm > cytosol. Nucleus. Secreted. Stimulation with IL1B leads to colocalization with SMAD3 mostly in perinuclear regions. Only the CASP1-cleaved mature form translocates into the nucleus upon LPS stimulation.
  • Information by UniProt
  • Alternative names

    • FIL1
    • FIL1 zeta
    • FIL1(ZETA)
    • FIL1Z
    • IL 1 zeta
    • IL 1F7
    • IL 1F7b (IL 1H4, IL 1H, IL 1RP1)
    • IL 1H4
    • IL 1RP1
    • IL 1X
    • IL 1X protein
    • IL-1 zeta
    • IL-1F7
    • IL-1H
    • IL-1H4
    • IL-1RP1
    • IL-1X
    • IL-37
    • IL1F7
    • IL1F7 (canonical product IL 1F7b)
    • IL1F7_HUMAN
    • IL1H4
    • IL1RP1
    • Interleukin 1 family member 7
    • interleukin 1 family, member 7 (zeta)
    • Interleukin 1 homolog 4
    • Interleukin 1 related protein
    • Interleukin 1 superfamily z
    • Interleukin 1 zeta
    • Interleukin 37
    • Interleukin-1 family member 7
    • Interleukin-1 homolog 4
    • Interleukin-1 zeta
    • Interleukin-1-related protein
    • Interleukin-23
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human IL-37 ELISA Kit (ab213798) Standard Curve.

Protocols

References

ab213798 has not yet been referenced specifically in any publications.

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