Overview

  • Product name

    Human IL-4 ELISA Kit
    See all IL-4 kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 5 pg/ml
  • Range

    3.13 pg/ml - 200 pg/ml
  • Recovery

    96 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 101.43 85% - 106%
    Serum 94.58 83% - 103%
    Plasma 96.24 84% - 104%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s IL-4 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-4 in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human IL-4 coated on a 96-well plate. Standards and samples are pipette into the wells and IL-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human IL-4 2 vials
    IL-4 Microplate (12 x 8 wells) 1 unit
    Recombinant Human IL-4 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Relevance

    IL-4 is a pleiotropic cytokine produced by activated T cells. It is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of IL-4. IL-4, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL-4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of IL-4 encoding distinct isoforms have been reported.
  • Cellular localization

    Secreted
  • Alternative names

    • B cell growth factor 1
    • B cell stimulatory factor 1
    • BCGF 1
    • BCGF1
    • Binetrakin
    • BSF 1
    • BSF-1
    • BSF1
    • IL 4
    • IL4
    • Interleukin 4
    • Lymphocyte stimulatory factor 1
    • MGC79402
    • Pitrakinra
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100570

  • Representative standard curve using ab100570

Protocols

References

This product has been referenced in:

  • Okamgba OC  et al. Variations in the leukocyte and cytokine profiles between placental and maternal circulation in pregnancy-associated malaria. Res Rep Trop Med 9:1-8 (2018). Read more (PubMed: 30050350) »
  • Hosseinkhani B  et al. Extracellular Vesicles Work as a Functional Inflammatory Mediator Between Vascular Endothelial Cells and Immune Cells. Front Immunol 9:1789 (2018). Read more (PubMed: 30131806) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

I have broken down the cross-reactivity information for each kit below:


· ab100549 IL10 Human ELISA Kit
o The capture antibody did not show any cross-reactivity with Murine IL-10 from testing. Thus, this kit is not expected to cross react with mouse samples.
· ab100566 IL2 Human ELISA Kit
o The capture antibody did not show any cross-reactivity with Murine IL-2 from testing. Thus, this kit is not expected to cross react with mouse samples.
· ab100570 IL4 Human ELISA Kit
o Capture Antibody
§ Mouse, monoclonal
§ Not tested for cross-reactivity with mouse samples.
o Detection Antibody
§ Goat, polyclonal
§ Detects the region His25­Ser153 (Accession # NP_000580)
§ Not tested for cross-reactivity with mouse samples.
· ab100689 Interferon gamma Mouse ELISA Kit
o Capture Antibody
§ Host species: Rat
§ Not tested for cross-reactivity with human samples.
o Detection Antibody
§ Host species: Rat
§ Not tested for cross-reactivity with human samples.
· ab100697 IL10 Mouse ELISA Kit
o Capture Antibody
§ Rat, monoclonal
§ Not tested for cross-reactivity with human samples.
o Detection Antibody
§ Rat, monoclonal
§ Not tested for cross-reactivity with human samples.
· ab100706 IL2 Mouse ELISA Kit
o The detection antibody did not show any cross-reactivity with Human IL-2 from testing. Thus, this kit is not expected to cross react with human samples.
· ab100710 IL4 Mouse ELISA Kit
o The capture antibody did not show any cross-reactivity with Human IL-4 from testing. Thus, this kit is not expected to cross react with human samples.

Read More

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