Overview

  • Product name

    Human IL-4 ELISA Kit
    See all IL-4 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    sample 6 3.5%
    Inter-assay
    Sample n Mean SD CV%
    sample 6 7.3%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 0.5 pg/ml
  • Range

    1.1 pg/ml - 35 pg/ml
  • Recovery

    107 %

    Sample specific recovery
    Sample type Average % Range
    Serum 100 75% - 121%

  • Assay time

    3h 50m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s IL-4 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-4 in supernatants, buffered solutions, serum and plasma samples.

    A monoclonal antibody specific IL-4 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-4 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL4 present in the samples. 

    This kit will recognize both endogenous and recombinant Human IL-4.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated anti IL-4 1 x 400µl 2 x 400µl
    Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    Control 2 vials 4 vials
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IL-4 Microplate (12 x 8 well strips) 1 unit 2 units
    IL-4 Standard (Lyophilized) 2 vials 4 vials
    Standard Diluent (Serum) 1 x 7ml 2 x 7ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • Research areas

  • Relevance

    IL-4 is a pleiotropic cytokine produced by activated T cells. It is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of IL-4. IL-4, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL-4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of IL-4 encoding distinct isoforms have been reported.
  • Cellular localization

    Secreted
  • Alternative names

    • B cell growth factor 1
    • B cell stimulatory factor 1
    • BCGF 1
    • BCGF1
    • Binetrakin
    • BSF 1
    • BSF-1
    • BSF1
    • IL 4
    • IL4
    • Interleukin 4
    • Lymphocyte stimulatory factor 1
    • MGC79402
    • Pitrakinra
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab46058

Protocols

References

ab46058 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab46058.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up