• Product name
    Human IL-4 ELISA Set (without plates)
    See all IL-4 kits
  • Sample type
    Cell culture supernatant, Serum, Plasma, Other biological fluids
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 0.5 pg/ml
  • Range
    35 pg/ml - 1.1 pg/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-4 Human in vitro ELISA Set is designed for the quantitative measurement of IL-4 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.


    The IL-4 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.


    This ELISA set is intended to measure human IL-4 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

    Recognizes both natural and recombinant human IL-4 Sensitivity: < 0.7 pg / ml

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 5 x 96 tests 10 x 96 tests 15 x 96 tests
    Detection biotinylated anti-IL-4 antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml
    IL-4 Capture antibody 1 x 0.1ml 2 x 0.1ml 3 x 0.1ml
    IL-4 Standard (Lyophilized) 5 vials 10 vials 15 vials
    Ready-to-use TMB 2 vials 4 vials 6 vials
    Streptavidin-HRP 1 vial 2 vials 3 vials
  • Research areas
  • Relevance
    IL-4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL-4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
  • Cellular localization
  • Alternative names
    • B cell stimulatory factor 1
    • Binetrakin
    • BSF1
    • IL4
    • Interleukin 4
    see all
  • Database links


Our Abpromise guarantee covers the use of ab48474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • IL-4 Standard curve ranging from 1.1 to 35 pg/mL


ab48474 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Unfortunately, the specific antibody pair in ab48474 is not available for sale separately. However, we do have 2 anti-IL4 primary antibodies that have been tested and are guaranteed to detect IL4 in a sandwich ELISA format for 12 months after purchase. I have listed this antibody pair below:

ab9622: https://www.abcam.com/il4-antibody-ab9622.html
ab9323: https://www.abcam.com/il4-antibody-ab9323.html

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The lab recommends the following for samples human serum or plasmas : 1/2 (50µl sample + 50µl diluent) and multiply the calculated concentration by 2. Regarding cell supernatants, expected values are very dependent to the cells assayed; customers usually make serial several dilution (1/2; 1/20; 1/200) rather than assaying one dilution in duplicate

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