Human IL-4 ELISpot Pair (ab281989)
Key features and details
- Assay type: ELISPOT
- Detection method: Colorimetric
- Sample type: Suspension cells
- Reacts with: Human
Overview
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Product name
Human IL-4 ELISpot Pair -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 12 6.5% Overall 12 2.2% Overall 12 6.8% Overall 12 6.6% Overall 12 2.5% -
Sample type
Suspension cells -
Assay type
ELISPOT -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-4 ELISpot Antibody Pair (ab281989) ) is an antibody pair designed for of cytokine and other soluble molecule production and secretion from T-cells at a single cell level by the ELISpot method.
For the quantitative determination of the frequency of Human IL-4 -producing cells.
For research use only - not intended for diagnostic use.
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Tested applications
Suitable for: ELISpotmore details -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 10 x 96 tests Biotinylated Human IL-4 detection antibody 2 vials Human IL-4 Capture Antibody 2 x 0.5ml -
Research areas
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Relevance
IL-4 is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. IL-4, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL-4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. -
Cellular localization
Secreted -
Alternative names
- B cell growth factor 1
- B cell IgG differentiation factor
- B cell stimulatory factor 1
see all -
Database links
- Entrez Gene: 3565 Human
- Omim: 147780 Human
- SwissProt: P05112 Human
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab281989 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISpot |
Use at an assay dependent concentration.
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Notes |
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ELISpot
Use at an assay dependent concentration. |
Datasheets and documents
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Datasheet download
References (0)
ab281989 has not yet been referenced specifically in any publications.