Human IL-5 ELISA Kit (ab100571)
- Datasheet
- References
- Protocols
Overview
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Product name
Human IL-5 ELISA Kit
See all IL-5 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 5 pg/ml -
Range
2.74 pg/ml - 2000 pg/ml -
Recovery
95 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 94.83 84% - 103% Serum 95.46 83% - 103% Plasma 96.32 84% - 104% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s IL-5 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-5 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human IL-5 coated on a 96-well plate. Standards and samples are pipette into the wells and IL-5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimization may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human IL-5 (lyophilized) 2 vials IL-5 Microplate (12 x 8 wells) 1 unit Recombinant Human IL-5 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells. -
Sequence similarities
Belongs to the IL-5 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- B cell differentiation factor I
- B-cell differentiation factor I
- Colony stimulating factor eosinophil
see all -
Database links
- Entrez Gene: 3567 Human
- Omim: 147850 Human
- SwissProt: P05113 Human
- Unigene: 2247 Human
Images
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Typical standard curveRepresentative standard curve using ab100571
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Typical standard curveRepresentative standard curve using ab100571
Protocols
References
ab100571 has not yet been referenced specifically in any publications.
