Human IL-5 ELISA Kit (ab100571)

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Typical standard curve
  • Typical standard curve

Key features and details

  • Sensitivity: 5 pg/ml
  • Range: 2.74 pg/ml - 2000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human IL-5 ELISA Kit
    See all IL-5 kits

  • Detection method

    Colorimetric

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    < 5 pg/ml

  • Range

    2.74 pg/ml - 2000 pg/ml

  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.83 84% - 103%
    Serum 95.46 83% - 103%
    Plasma 96.32 84% - 104%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam’s IL-5 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-5 in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human IL-5 coated on a 96-well plate. Standards and samples are pipette into the wells and IL-5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Platform

    Microplate

Properties

Associated products

    Images

    • Typical standard curve
      Typical standard curve

      Representative standard curve using ab100571

    • Typical standard curve
      Typical standard curve

      Representative standard curve using ab100571

    References (0)

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