Human IL-5 ELISA Set (without plates) (ab47356)
Overview
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Product name
Human IL-5 ELISA Set (without plates) -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 5 pg/ml -
Range
7.8 pg/ml - 250 pg/ml -
Assay time
3h 45m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s IL-5 Human in vitro ELISA Set is designed for the quantitative measurement of IL-5 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
This ELISA Set is intended to measure human IL-5 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
These reagents are intended for research use only, not for diagnostic purposes.
The IL-5 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
Recognizes both natural and recombinant human IL-5.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 10 x 96 tests 5 x 96 tests 15 x 96 tests Chromogen TMB Substrate Solution 4 vials 2 vials 6 vials Detection biotinylated anti-IL-5 antibody 2 x 0.55ml 1 x 0.55ml 3 x 0.55ml IL-5 Capture antibody 2 x 0.5ml 1 x 0.5ml 3 x 0.5ml IL-5 standard Yellow 10 vials 5 vials 15 vials Streptavidin-HRP 2 vials 1 vial 3 vials -
Research areas
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Relevance
Interleukin 5 (IL-5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL2, IL4 and growth hormone. Human and mouse IL5 have 70% amino acid sequence homology. -
Cellular localization
Secreted -
Alternative names
- B cell differentiation factor I
- Eosinophil differentiation factor
- IL5
see all -
Database links
- Entrez Gene: 3567 Human
- SwissProt: P05113 Human
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Sandwich ELISA |
Use at an assay dependent concentration.
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Notes |
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Sandwich ELISA
Use at an assay dependent concentration. |
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab47356 has not yet been referenced specifically in any publications.