Overview

  • Product name
    Human IL-5 ELISA Set (without plates)
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 5 pg/ml
  • Range
    7.8 pg/ml - 250 pg/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-5 Human in vitro ELISA Set is designed for the quantitative measurement of IL-5 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.


     


    This ELISA Set is intended to measure human IL-5 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.


    These reagents are intended for research use only, not for diagnostic purposes.


     


    The IL-5 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.


     


    Recognizes both natural and recombinant human IL-5.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Reagents

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 5 x 96 tests 10 x 96 tests 15 x 96 tests
    Detection biotinylated anti-IL-5 antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml
    IL-5 Capture antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml
    IL-5 standard Yellow 5 vials 10 vials 15 vials
    Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials
    Streptavidin-HRP 1 vial 2 vials 3 vials
  • Research areas
  • Relevance
    Interleukin 5 (IL-5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL2, IL4 and growth hormone. Human and mouse IL5 have 70% amino acid sequence homology.
  • Cellular localization
    Secreted
  • Alternative names
    • B cell differentiation factor I
    • Eosinophil differentiation factor
    • IL5
    • Interleukin 5
    • T cell replacing factor
    • TRF
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab47356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • IL-5 Standard curve ranging from 7.8 to 250 pg/mL

References

ab47356 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab47356.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up