Overview

  • Product name

    Human IL-6 ELISA Kit
    See all IL-6 kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 3 pg/ml
  • Range

    1.37 pg/ml - 1000 pg/ml
  • Recovery

    94 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.65 83% - 105%
    Serum 92.63 83% - 103%
    Plasma 93.74 84% - 104%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s IL-6 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-6 in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human IL-6 coated on a 96-well plate. Standards and samples are pipette into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get higher sensitivity in only 90 minutes with Human IL-6 ELISA Kit (ab178013) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    600X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human IL-6 (lyophilized) 2 vials
    IL-6 Microplate (12 x 8 wells) 1 unit
    Recombinant Human IL-6 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Function

    Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • Involvement in disease

    Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Sequence similarities

    Belongs to the IL-6 superfamily.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Interleukin BSF 2
    • B cell differentiation factor
    • B cell stimulatory factor 2
    • B-cell stimulatory factor 2
    • BSF 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • Cytotoxic T cell differentiation factor
    • Hepatocyte stimulating factor
    • Hepatocyte stimulatory factor
    • HGF
    • HSF
    • Hybridoma growth factor
    • Hybridoma growth factor Interferon beta-2
    • Hybridoma plasmacytoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL 6
    • IL-6
    • IL6
    • IL6_HUMAN
    • Interferon beta 2
    • Interferon beta-2
    • Interleukin 6
    • Interleukin 6 (interferon beta 2)
    • Interleukin BSF 2
    • Interleukin-6
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab100572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100572

  • Representative standard curve using ab100572

Protocols

References

This product has been referenced in:

  • Giles KM  et al. microRNA-7-5p inhibits melanoma cell proliferation and metastasis by suppressing RelA/NF-?B. Oncotarget 7:31663-80 (2016). Read more (PubMed: 27203220) »
  • Hong M  et al. Anti-inflammatory and antifatigue effect of Korean Red Ginseng in patients with nonalcoholic fatty liver disease. J Ginseng Res 40:203-10 (2016). Read more (PubMed: 27616896) »
See all 4 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

Thank you for your inquiry.
Do you have the catalogue numbers of the kits you're inquiring about? We have 7 different IL6 Human ELISA kits in the catalogue: ab46044, ab46042, ab46027, ab46041, ab100572, ab100573 and ab108867.
The IL6 High Sensitivity kits (ab46044 and ab46042) are the same, just one is 1 x 96 tests and one is 2 x 96 tests. These are high sensitivity at < 0.8 pg/ml and is in sandwich format and works with cell culture supernatant, Serum, and Plasma.
The ab46027 works with Cell culture supernatant, Serum, and Plasma as well in a sandwich format, but the sensitivity is < 2 pg/ml, but greater recovery.
The ab100572 has a broader range 1.37 pg/ml - 1000 pg/ml but < 3 pg/ml sensitivity. This works in Cell culture supernatant, Serum, and Plasma as well.
The ab100573 is the same as ab100572, except that ab100573 is recommended for cell culture extracts and tissue extracts.
The ab1008867 works with works with Cell culture supernatant, Serum, and Plasma as well in a sandwich format but is the least sensitive (8 pg/ml).
If you purchase multiple kits, you will only pay 1 shipping charge. If an item on your order is out of stock, your order will be on hold until it is ready to ship. However, you can choose to "split" your order to receive the in-stock item the next day, and the out of stock item when it comes in, but you would have to pay for both shipping charges in that case.
I hope this information helps. Please contact us with any other questions.

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Answer

My colleague is out of office for the rest of the week.

As I understand from your previous e-mail, due to malfunction of the refrigerator this kit failed to work. Is that correct, would you be so kind to confirm if this is the case?

Though you have kindly provided some details, it would be much appreciated if I could get some more information which would help me identify the source of the problem.



Q1: Could explain further the internal temperature measured/recorded in the refrigerator?

Q2: Has the customer already used the kit?

Q3: Are the serum samples normal of from pathological conditions?



I look forward to hearing from you soon.

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Answer

Thank you for your enquiry.

As the on-line datasheets indictae ab100573 is suiatbel for cell culture extracts, tissue extracts, whilst ab100572 for cell culture supernatant, urine, serum, plasma.

I would advise you totake a look at teh Protocol Booklets for these two kist which are available at our website:

https://www.abcam.com/ps/products/100/ab100572/documents/IL6-Human-ELISA-Kit-ab100572-protocol-plain.pdf

https://www.abcam.com/ps/products/100/ab100573/documents/IL6-Human-ELISA-Kit-ab100573-protocol.pdf

I hope this helps and if I can assist further, please do not hesitate to contact me.

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Answer

Thank you for your interest in our IL6 and TNF alpha ELISA kits. My colleague will be happy to discuss pricing with you. As I mentioned, she will ask how many kits you want of each type, IL6 and TNF alpha. If you have any technical questions, our Scientific Support team is available from 8:30 in the morning to 8:00 in the evenening, EST.  

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Answer

Thank you for your email. We have ab100647 in stock. I have also requested our datasheet department for addition of a ab65355 standard as separate product in catalogue. I am now waiting a reply from them. I will be updating you further once this is done. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Thank you for your below email and support Kindly find hereby in green the answers  we have just received from our customer, as well as our comments marked in red - Could you please ask customer to provide step by step protocol details they have used? We followed the exact protocol as indicated in the datasheets Kindly note that The customer is suspicious about the protocol of the TGF-Beta which was amended following your e-mail dated August 11, 2011 Subject: Important information regarding ab100647 [CCE3054067] and is complaining that the problem in his OD readings is due to the difference in  the protocol, same for cAMP standards , they had a problem with the OD readings , how do you explain it? - Is it true the HRP-streptavidin vials in kit ab46087 and ab100647 were completely empty? The streptavidin vials of both kits were totally empty Kindly advice what might have affected the HRP vials, and why the small volume (8ul) was not present, is it possible for the HRP to evaporate under specific conditions? - If the streptavidine-HRP was missing in ab100647; how the experiment was performed? For the ab46087 and ab100647 kits, we utilized the streptavidine of the IL 6 kit - Step 8 is very crucial in protocol of ab65355; could you please ask customer how they performed this step? We performed step 8 exactly as stated in the manual Add 5 microliters of acetylating reagent in each tube and vortex immediately 2 to 3 seconds from tube to tube and incubate 10 minutes at room temperature. This was followed exactly - Could you send us the OD readings of experiment performed? Attached you will find a copy of the relative ODs (TGF-Beta and cAMP readings) Looking forward to receive your reply ASAP, to solve this issue Best regards

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Answer

Thank you for answeirng my questions. Ab10647 have HRP standard 8ul vial and ab46087 have 2 vial of 4ul each. The amount of HRP-Streptavidin in these vials is very-very small; might not be visible. We would recommend following the datasheets reconstituting these further in working volume. For example in case of ab46087 we recommend "Diluting the Streptavidin-HRP 1:100 just prior to use by adding 0.5 ml of HRP diluent to the vial containing Streptavidin-HRP concentrate and for ab100647 we recommend briefly spinning the HRP-Streptavidin concentrate vial (Item G) and Pipetting up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 25,000-fold with 1x Assay Diluent B. The solution is not evaporated but the amount is very less which is hardly visible so I would recommend following the protocol as recommended on the datasheets. I am happy in sending a free of charge replacement for ab100647 and a vial of standard for ab65355. Please confirm if you are happy in receiving it. Please use the standards as provided for ab100572 and ab46087; these kits will be fine. Looking forward to hearing from you soon.

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Answer

Thank you for contacting us. I apologize for the delay in response. I will try to solve this case asap. I have read your email which is slightly confusing so I would appreciate if you could answer my following questions. - Could you please ask customer to provide step by step protocol details they have used? - Is it true the HRP-streptavidin vials in kit ab46087 and ab100647 were completely empty? - If the streptavidine-HRP was missing in ab100647; how the experiment was performed? - Step 8 is very crucial in protocol of ab65355; could you please ask customer how they performed this step? - Could you send us the OD readings of experiment performed? ab46087, ab100572 and ab100647 have expiry date of 1 year if stored under conditions as recommended on the datasheet. I will look forward to hearing from you soon.

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