Human IL-6 ELISA Kit (ab100573)
Key features and details
- Sensitivity: 30 pg/ml
- Range: 1.37 pg/ml - 1000 pg/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-6 ELISA Kit
See all IL-6 kits -
Detection method
Colorimetric -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
< 30 pg/ml -
Range
1.37 pg/ml - 1000 pg/ml -
Recovery
93 %
Sample specific recovery Sample type Average % Range Cell culture extracts 93.65 82% - 102% Tissue Extracts 92.78 81% - 101% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Human IL-6 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-6 in cell lysates and tissue lysates.
This assay employs an antibody specific for Human IL-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human IL-6 ELISA Kit (ab178013) from our SimpleStep ELISA® range.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 2X Cell Lysis Buffer 1 x 5ml 5X Assay Diluent 1 x 15ml 5X Sample Diluent Buffer 1 x 10ml 600X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated anti-Human IL-6 2 vials IL-6 Microplate (12 x 8 wells) 1 unit Recombinant Human IL-6 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. -
Involvement in disease
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Interleukin BSF 2
- B cell differentiation factor
- B cell stimulatory factor 2
see all -
Database links
- Entrez Gene: 3569 Human
- Omim: 147620 Human
- SwissProt: P05231 Human
- Unigene: 654458 Human
Associated products
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SimpleStep ELISA kits
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab100573 has been referenced in 4 publications.
- Huang P & Jia L MicroRNA-28-5p as a potential diagnostic biomarker for chronic periodontitis and its role in cell proliferation and inflammatory response. J Dent Sci 17:1501-1509 (2022). PubMed: 36299340
- Du YX et al. Development of a dendrimer PAMAM-based gold biochip for rapid and sensitive detection of endogenous IFN-? and anti-IFN-? IgG in patients with hemophagocytic lymphohistiocytosis. Mol Med Rep 22:5369-5377 (2020). PubMed: 33173980
- Chen Z et al. Downregulation of miR-199a-3p mediated by the CtBP2-HDAC1-FOXP3 transcriptional complex contributes to acute lung injury by targeting NLRP1. Int J Biol Sci 15:2627-2640 (2019). PubMed: 31754335
- Zhang N et al. Dapagliflozin improves left ventricular remodeling and aorta sympathetic tone in a pig model of heart failure with preserved ejection fraction. Cardiovasc Diabetol 18:107 (2019). PubMed: 31429767