Overview

  • Product name
    Human IL-6 ELISA Kit 
    See all IL-6 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Pooled sampl 15 4.2%
    Inter-assay
    Sample n Mean SD CV%
    Sample 1 18 200pg/ml 16 8.1%
    Sample 2 18 77pg/ml 8 10.1%
    Sample 3 18 63pg/ml 6 8.6%
    Sample 4 18 103pg/ml 6 6%
    Sample 5 18 53pg/ml 3 5.5%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 2 pg/ml
  • Range
    6.25 pg/ml - 200 pg/ml
  • Recovery

    96 %

  • Assay time
    1h 45m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-6 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-6 in Human serum, plasma, buffered solutions or supernatants.


    A monoclonal antibody specific for IL-6 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-6 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-6 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-6 present in the samples.


    This kit will recognize both endogenous and recombinant Human IL-6.


    Get higher sensitivity in only 90 minutes with Human IL-6 ELISA Kit (ab178013) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Adhesive plate seal 1 x 2 units 1 x 4 units
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Biotinylated anti-IL-6 Red 1 x 0.4ml 2 x 0.4ml
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IL-6 Control Silver 2 vials 4 vials
    IL-6 Microplate (12 x 8 well strips) 1 unit 2 units
    IL-6 Standard (Lyophilized) Yellow 2 vials 4 vials
    Standard Diluent (Serum) 1 x 7ml 2 x 7ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • Research areas
  • Function
    Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • Involvement in disease
    Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Sequence similarities
    Belongs to the IL-6 superfamily.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • Interleukin BSF 2
    • B cell differentiation factor
    • B cell stimulatory factor 2
    • B-cell stimulatory factor 2
    • BSF 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • Hepatocyte stimulatory factor
    • HGF
    • HSF
    • Hybridoma growth factor
    • Hybridoma growth factor Interferon beta-2
    • IFN-beta-2
    • IFNB2
    • IL 6
    • IL-6
    • IL6
    • IL6_HUMAN
    • Interferon beta 2
    • Interferon beta-2
    • Interleukin 6
    • Interleukin 6 (interferon beta 2)
    • Interleukin BSF 2
    • Interleukin-6
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab46027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Standard curve of human IL-6 with background signal subtracted (duplicates; +/- SD).

  • IL-6 measured in cell culture supernatants (diluted 1/50-1/1250) from control or treated (48 hours with 2% PHA-M; LifeTechnologies) PBMCs (duplicates +/- SD).

  • IL-6 detected in positive control (Pos CTRL; ab46027), and in supernatants (diluted 1/1-1/5) from control cells or cells stimulated for 24 hours with 50 nanogram x mL-1 of PMA (ab120297) with 1 microgram  x mL-1 of LPS (Sigma) for the last 6 hours (PMA/LPS).

  • Representative Standard Curve using ab46027

  • Dilution curves of human IL-6 (ab46027) and NIBSC standard (89/548). One ng of standard IL-6 corresponds to 87.2 (+/- 8.0) IU NIBSC standard. Background signal subtracted (duplicates; +/- SD).

Protocols

References

This product has been referenced in:
  • Yamamoto M  et al. Stage classification of IgG4-related dacryoadenitis and sialadenitis by the serum cytokine environment. Mod Rheumatol N/A:1-5 (2018). Read more (PubMed: 29385874) »
  • Yu S  et al. Mediating the invasion of smooth muscle cells into a cell-responsive hydrogel under the existence of immune cells. Biomaterials 180:193-205 (2018). Read more (PubMed: 30048909) »
See all 8 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Question
Answer

Thank you for contacting us.

Your buffer should be fine if you can keep the pH to around 7.0 for protein staibility.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your inquiry.
Do you have the catalogue numbers of the kits you're inquiring about? We have 7 different IL6 Human ELISA kits in the catalogue: ab46044, ab46042, ab46027, ab46041, ab100572, ab100573 and ab108867.
The IL6 High Sensitivity kits (ab46044 and ab46042) are the same, just one is 1 x 96 tests and one is 2 x 96 tests. These are high sensitivity at < 0.8 pg/ml and is in sandwich format and works with cell culture supernatant, Serum, and Plasma.
The ab46027 works with Cell culture supernatant, Serum, and Plasma as well in a sandwich format, but the sensitivity is < 2 pg/ml, but greater recovery.
The ab100572 has a broader range 1.37 pg/ml - 1000 pg/ml but < 3 pg/ml sensitivity. This works in Cell culture supernatant, Serum, and Plasma as well.
The ab100573 is the same as ab100572, except that ab100573 is recommended for cell culture extracts and tissue extracts.
The ab1008867 works with works with Cell culture supernatant, Serum, and Plasma as well in a sandwich format but is the least sensitive (8 pg/ml).
If you purchase multiple kits, you will only pay 1 shipping charge. If an item on your order is out of stock, your order will be on hold until it is ready to ship. However, you can choose to "split" your order to receive the in-stock item the next day, and the out of stock item when it comes in, but you would have to pay for both shipping charges in that case.
I hope this information helps. Please contact us with any other questions.

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Answer

Thank you very much for contacting us with your questions.

The wells will come as 12 strips of 8 wells each, so you can remove as many strips as you need from the pouch immediately before use, then store the remainingstrips in the pouch with dessicant. Make sure the pouch is sealed tightly and return it to storage at 4C.

Fordata analysis, you will need to calculate the average absorbance values and create a standard curve, then interpolate the sample concentrations from the standard curve. These steps can be done in Excel.

I hope this information will be useful, but if you have any further questions or if there is anything else that we can do for you, please let me know and I'll be happy to help.

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Answer

Thank you for contacting us.

All of the kits mentioned (ab46027, ab100512, ab100640, ab108891) are suitable for use with citrate plasma.

I hope this information has been of help. If your require any further information please do not hesitate to contact us again.

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Answer

Thank you for contacting us. I apologise for the delay.
Unfortunately any of these kits have been tested with human ascites. However, they should work as they are suitable for all body fluids.
The only problem I would suspect is the high concentration of IgG in ascites which can interfere with the ELISA. I would suggest diluting samples 1:2 or 1:5 to minimize the matrix effect.
I hope this helps and if we can assist further, please do not hesitate to contact us.

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Question
Answer

Thank you for contacting us. Could you write more information about your question? Looking forward to hearing from you soon.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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