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    human-il-6-elisa-kit-high-sensitivity-ab46042.pdf

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Human IL-6 ELISA Kit High Sensitivity (ab46042)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (3)Q&A (6)References (55)

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Sandwich ELISA - IL-6 High Sensitivity Human ELISA Kit (ab46042)
  • Typical Standard Curve for ab46042 Human IL-6 ELISA kit - High sensitivity

Key features and details

  • Sensitivity: 0.8 pg/ml
  • Range: 1.56 pg/ml - 50 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

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Overview

  • Product name

    Human IL-6 ELISA Kit High Sensitivity
    See all IL-6 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.4%
    Inter-assay
    Sample n Mean SD CV%
    Sample 1 18 25pg/ml 3 12.2%
    Sample 2 18 12pg/ml 1 8.7%
    Sample 3 18 6pg/ml 1 13.7%
    Sample 4 18 41pg/ml 2 5.9%
    Sample 5 18 31pg/ml 1 4.9%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 0.8 pg/ml
  • Range

    1.56 pg/ml - 50 pg/ml
  • Recovery

    93 %

  • Assay time

    3h 45m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human IL-6 ELISA kit - High sensitivity is a sandwich ELISA kit for the quantitative measurement of IL-6 in supernatants, buffered solutions, serum, plasma and other body fluids. This assay will recognise both natural and recombinant Human IL-6.


    A monoclonal antibody specific for IL-6 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-6 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-6 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-6 present in the samples. 


    This kit will recognize both endogenous and recombinant Human IL-6.


    Get results in 90 minutes with Human IL-6 ELISA Kit (ab178013) from our SimpleStep ELISA® range.


     

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Biotinylated anti-IL-6 Red 1 x 0.4ml 2 x 0.4ml
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 12ml 1 x 23ml
    IL-6 HS Control Yellow 2 vials 4 vials
    IL-6 HS Standard (Lyophilized) Orange 2 vials 4 vials
    IL-6 Microplate (12 x 8 well strips) 1 unit 2 units
    Plastic Plate Covers 1 x 2 units 1 x 4 units
    Standard Diluent (Serum) 1 x 7ml 2 x 7ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Neuroscience
    • Neurology process
    • Metabolism
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interleukins
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cytokines and cytokine receptors ELISA kits
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Metabolic disorders
    • Neuroscience
    • Processes
  • Function

    Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • Involvement in disease

    Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Sequence similarities

    Belongs to the IL-6 superfamily.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P05231 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Interleukin BSF 2
    • B cell differentiation factor
    • B cell stimulatory factor 2
    • B-cell stimulatory factor 2
    • BSF 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • Cytotoxic T cell differentiation factor
    • Hepatocyte stimulating factor
    • Hepatocyte stimulatory factor
    • HGF
    • HSF
    • Hybridoma growth factor
    • Hybridoma growth factor Interferon beta-2
    • Hybridoma plasmacytoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL 6
    • IL-6
    • IL6
    • IL6_HUMAN
    • Interferon beta 2
    • Interferon beta-2
    • Interleukin 6
    • Interleukin 6 (interferon beta 2)
    • Interleukin BSF 2
    • Interleukin-6
    see all
  • Database links

    • Entrez Gene: 3569 Human
    • Omim: 147620 Human
    • SwissProt: P05231 Human
    • Unigene: 654458 Human

    Associated products

    • SimpleStep ELISA kits

      • Human IL-6 ELISA Kit (ab178013)

    Images

    • Sandwich ELISA - IL-6 High Sensitivity Human ELISA Kit (ab46042)
      Sandwich ELISA - IL-6 High Sensitivity Human ELISA Kit (ab46042)

      IL-6 was measured in cell culture medium from U937 control cells, or cells stimulated with LPS (1 µg x mL-1; 6 h) or PMA (10 ng x mL-1; 24 h) and LPS (background signal subtracted) (duplicates +/- SD).

    • Typical Standard Curve for ab46042 Human IL-6 ELISA kit - High sensitivity
      Typical Standard Curve for ab46042 Human IL-6 ELISA kit - High sensitivity

      Representative Standard Curve using ab46042

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (55)

    Publishing research using ab46042? Please let us know so that we can cite the reference in this datasheet.

    ab46042 has been referenced in 55 publications.

    • Lei Z  et al. Long non-coding RNA maternally expressed gene regulates cigarette smoke extract induced lung inflammation and human bronchial epithelial apoptosis via miR-149-3p. Exp Ther Med 21:60 (2021). PubMed: 33365060
    • Yang X  et al. Zoledronic acid re-sensitises gefitinib-resistant lung cancer cells by inhibiting the JAK/STAT3 signalling pathway and reversing epithelial-mesenchymal transition. Oncol Rep 45:459-468 (2021). PubMed: 33416163
    • Khor BH  et al. Circulating fatty acid profiles are associated with protein energy wasting in maintenance hemodialysis patients: a cross-sectional study. Sci Rep 11:1416 (2021). PubMed: 33446880
    • Ma YS  et al. M2 macrophage-derived exosomal microRNA-155-5p promotes the immune escape of colon cancer by downregulating ZC3H12B. Mol Ther Oncolytics 20:484-498 (2021). PubMed: 33718596
    • Qin G  et al. LncRNA NR024118 is downregulated in sepsis and inhibits LPS-induced apoptosis of cardiomyocytes. Mol Med Rep 23:N/A (2021). PubMed: 33846787
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-9 of 9 Abreviews or Q&A

    Amazing kit

    Excellent Average 3/5 (Ease of Use)
    Abreviews
    Abreviews
    I have only recently started doing ELISA. This kit was very easy to do compared to some of the other ELISA I have done. The directions were well written and easy to follow. There was more than enough solution for everything I need. I would recommend to anyone 10/10.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 23 2020

    Senior Principal Scientist

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    The assay has adquate sensitivity for my application. My application was to evaluate IL-6 level of human blood after exposed to Foreign Materials (FM) observed in certain blood circulation medical devices. The high sensitivity of the assay is very critical for my application. The assay has good linearity, it is very helpful.

    I also want to comment on the fast delivery of the product and easy of use for the product ordering on line.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Charlene Yuan

    Verified customer

    Submitted Aug 21 2014

    Abreview for IL6 High Sensitivity Human ELISA Kit

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image

    Sample: Human Cell (Monocytes)
    Specification: Monocytes
    Amount of Sample: 10 µg
    Type of Kit Used: ELISA
    Did you use the Abcam Kit protocol?: Yes
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 29 2013

    Question

    I have a few questions about this kit:
    1. Once the biotinilated antibody is reconstituted, how long can it be stored for? And at what temp should it be stored?
    2. What is the difference between the Standard and Control?
    3. Are both the Standard and Control vials lyophilized?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 16 2014

    Answer

    1. The Biotinylated Antibody is provided in liquid form, it is stable at 4°C
    2. Standards and Controls are provided lyophilized and should be reconstituted either by the volume stated on the vial or in the Protocol Booklet. They should be used directly once reconstituted and can't be stored.


    3. The Standard and Control are both made with recombinant IL-6, only the concentration differs.

    Read More

    Jeremy Kasanov

    Abcam Scientific Support

    Answered on Apr 16 2014

    Question

    What is the difference between the Interleukin 6 High Sensitivity Human ELISA kit and the Interleukin 6 Human ELISA kit. When ordering for the 2 products, do I have to pay seperately for shipping charges?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 11 2012

    Answer

    Thank you for your inquiry.
    Do you have the catalogue numbers of the kits you're inquiring about? We have 7 different IL6 Human ELISA kits in the catalogue: ab46044, ab46042, ab46027, ab46041, ab100572, ab100573 and ab108867.
    The IL6 High Sensitivity kits (ab46044 and ab46042) are the same, just one is 1 x 96 tests and one is 2 x 96 tests. These are high sensitivity at < 0.8 pg/ml and is in sandwich format and works with cell culture supernatant, Serum, and Plasma.
    The ab46027 works with Cell culture supernatant, Serum, and Plasma as well in a sandwich format, but the sensitivity is < 2 pg/ml, but greater recovery.
    The ab100572 has a broader range 1.37 pg/ml - 1000 pg/ml but < 3 pg/ml sensitivity. This works in Cell culture supernatant, Serum, and Plasma as well.
    The ab100573 is the same as ab100572, except that ab100573 is recommended for cell culture extracts and tissue extracts.
    The ab1008867 works with works with Cell culture supernatant, Serum, and Plasma as well in a sandwich format but is the least sensitive (8 pg/ml).
    If you purchase multiple kits, you will only pay 1 shipping charge. If an item on your order is out of stock, your order will be on hold until it is ready to ship. However, you can choose to "split" your order to receive the in-stock item the next day, and the out of stock item when it comes in, but you would have to pay for both shipping charges in that case.
    I hope this information helps. Please contact us with any other questions.

    Read More

    Abcam Scientific Support

    Answered on Oct 11 2012

    Question

    have saliva samples been tested with this kit?
    Do you have any general advice

    Read More

    Abcam community

    Verified customer

    Asked on Aug 15 2012

    Answer

    Thank you for contacting us.

    The lab let me know the following:
    Saliva is suitable for thisELISA kit, and the regular protocol would apply. There is no need to pre-treat the samples before assaying.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Aug 15 2012

    Question

    Can you please check if the below kits can be used for biological fluids , i.e in Urine ?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 16 2012

    Answer

    Thanks for your enquiry.



    Those kits are suitable for all body fluids, so urine is applicable.






    I hope this helps.

    Read More

    Abcam Scientific Support

    Answered on Apr 16 2012

    Question

    Dear Tech Support Team,

    Kindly advise if the following products can be used with human ascites:

    https://www.abcam.com/High-Sensitivity-IL6-Human-ELISA-Kit-1-x-96-Well-Plate-ab46042.html

    https://www.abcam.com/High-Sensitivity-IL6-Human-ELISA-Kit-2-x-96-Well-Plates-ab46044.html

    https://www.abcam.com/IL6-Human-Elipair-Kit-Reagents-for-10-x-96-Well-Plates-ab48479.html

    https://www.abcam.com/IL6-Human-Elipair-Kit-Reagents-for-5-x-96-Well-Plates-ab48478.html

    https://www.abcam.com/IL6-Human-ELISA-Kit-ab108867.html

    https://www.abcam.com/IL6-Human-ELISA-Kit-1-x-96-Well-Plate-ab46027.html

    https://www.abcam.com/IL8-Human-ELISA-Kit-1-x-96-Well-Plate-ab46032.html

    https://www.abcam.com/IL8-Human-ELISA-Kit-ab100575.html

    https://www.abcam.com/IL8-Human-ELISA-Kit-ab108869.html

    https://www.abcam.com/IL8-Human-ELISA-Kit-2-x-96-Well-Plates-ab46049.html

    https://www.abcam.com/IL8-Human-ELISA-Kit-1-x-96-Well-Plate-ab100576.html

    Thanks in advance for your assistance and reply.
    Best Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Feb 27 2012

    Answer

    Thank you for contacting us. I apologise for the delay.
    Unfortunately any of these kits have been tested with human ascites. However, they should work as they are suitable for all body fluids.
    The only problem I would suspect is the high concentration of IgG in ascites which can interfere with the ELISA. I would suggest diluting samples 1:2 or 1:5 to minimize the matrix effect.
    I hope this helps and if we can assist further, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    Answered on Feb 27 2012

    Question

    Dears,   I have a customer who want those kits to be working on Saliva Samples , Please confirm ASAP, thanks !   High Sensitivity IL6 Human ELISA Kit (1 x 96 Well Plate) (ab46042)   C Reactive Protein Human ELISA Kit (ab108826)   --   Kindest regards,

    Read More

    Abcam community

    Verified customer

    Asked on Dec 16 2011

    Answer

    Thank you for your enquiry. I can confirm that both these kits ab46042 and ab108826 should be suitable for use with saliva samples. I can confirm that  kit ab108826 has been tested on saliva samples,  We suggest to use saliva samples at 1x (no dilution) for this kit. We would suggest ab46042 should be suitable for saliva samples. However, this has not been specifically tested. Therefore, we are able to offer a discount for this kit in return for agreement to submit an Abreview with the data. I am currently waiting to hear from my colleagues how much of a discount we can offer and will be in touch with you as soon as possible. I hope this will be helpful. If you have any further questions, please do let me know. I will be in touch again regarding ab46052.  

    Read More

    Abcam Scientific Support

    Answered on Dec 16 2011

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