Overview

  • Product name
    Human IL-6 ELISA Kit High Sensitivity
    See all IL-6 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.4%
    Inter-assay
    Sample n Mean SD CV%
    Sample 1 18 25pg/ml 3 12.2%
    Sample 2 18 12pg/ml 1 8.7%
    Sample 3 18 6pg/ml 1 13.7%
    Sample 4 18 41pg/ml 2 5.9%
    Sample 5 18 31pg/ml 1 4.9%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 0.8 pg/ml
  • Range
    1.56 pg/ml - 50 pg/ml
  • Recovery

    93 %

  • Assay time
    3h 45m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-6 Human High Sensitivity in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-6 in supernatants, buffered solutions, serum, plasma and other body fluids. This assay will recognise both natural and recombinant Human IL-6.


    A monoclonal antibody specific for IL-6 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-6 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-6 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-6 present in the samples. 


    This kit will recognize both endogenous and recombinant Human IL-6.


    Get results in 90 minutes with Human IL-6 ELISA Kit (ab178013) from our SimpleStep ELISA® range.


     

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Biotinylated anti-IL-6 Red 1 x 0.4ml 2 x 0.4ml
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IL-6 HS Control Silver 2 vials 4 vials
    IL-6 Microplate (12 x 8 well strips) 1 unit 2 units
    IL-6 HS Standard (Lyophilized) Yellow 2 vials 4 vials
    Plastic Plate Covers 1 x 2 units 1 x 4 units
    Standard Diluent (Serum) 1 x 7ml 2 x 7ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • Research areas
  • Function
    Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • Involvement in disease
    Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Sequence similarities
    Belongs to the IL-6 superfamily.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • Interleukin BSF 2
    • B cell differentiation factor
    • B cell stimulatory factor 2
    • B-cell stimulatory factor 2
    • BSF 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • Hepatocyte stimulatory factor
    • HGF
    • HSF
    • Hybridoma growth factor
    • Hybridoma growth factor Interferon beta-2
    • IFN-beta-2
    • IFNB2
    • IL 6
    • IL-6
    • IL6
    • IL6_HUMAN
    • Interferon beta 2
    • Interferon beta-2
    • Interleukin 6
    • Interleukin 6 (interferon beta 2)
    • Interleukin BSF 2
    • Interleukin-6
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab46042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • IL-6 was measured in cell culture medium from U937 control cells, or cells stimulated with LPS (1 �g x mL-1; 6 h) or PMA (10 ng x mL-1; 24 h) and LPS (background signal subtracted) (duplicates +/- SD).
  • Representative Standard Curve using ab46042

Protocols

References

This product has been referenced in:
  • Yang Z  et al. miR-143-3p regulates cell proliferation and apoptosis by targeting IGF1R and IGFBP5 and regulating the Ras/p38 MAPK signaling pathway in rheumatoid arthritis. Exp Ther Med 15:3781-3790 (2018). Read more (PubMed: 29581736) »
  • Mayr HL  et al. Randomization to 6-month Mediterranean diet compared with a low-fat diet leads to improvement in Dietary Inflammatory Index scores in patients with coronary heart disease: the AUSMED Heart Trial. Nutr Res 55:94-107 (2018). Read more (PubMed: 29754829) »
See all 18 Publications for this product

Customer reviews and Q&As

11-15 of 15 Abreviews or Q&A

Question

Hi
below are the answers to all questions.
1. You mentioned thatyou used 3 strips. Were all of these using the standard provided with the kit? What results did you obtain with the other 2 strips? For the time , i did run only 3 strips ( 2 forstandardsand one strip for 4samples), there are the result which i had..
strip 1(standards)
strip 2 (standards)
strip 3 (samples in duplicate)
AND THE SECOND TIME I DID RUN ONLY 1 STRIP ( THE STANDARD ONLY ), i have emailed you the results that i had for this time.
2. Were all reagents allowed to come to room temperature before use? YES, all reagents allowed to room temperature before use.
3.Exactly how was the standard prepared and diluted? What diluent was used? because i used a human sample (serum) so i used the standerd diluent : human serum which is ready to use ( no need to dilute ).
4. When washing, was the excess liquid removed by aspiration or tapping plate on absorbent paper? Tapping the plate onabsorbentpaper
5. Was the TMB solution protected from the light? Yes it was in the kit's box, i only took it out of the box before use it and i did protect the plate from the light by wrapping it withaluminumfoil.
6. What colour was the TMB solution before adding to the wells?colorless
7. How was the biotinylated anti-IL-6 prepared? according to the no of stripes i need, so i did add 60µ of Biotinylated Antibody concentrate and 1590µ
of Biotinylated Antibody diluent in a clean glass tube.
8. How was the Streptavidin-HRP prepared? i add 200µ from Diluent and 2µ from HRP.
9. Were the strips covered during the 3 hour incubation of the biotinylated anti-IL6? YES they were covered.
10. Was the HRP solution prepared directly prior to use? yes it was prepared immediately before use.
11.At whatwavelength was the absorbancemeasured? What kind of reader have you been using? at 450 nm
12. The wash steps you refer to, after which steps werethese performed and exactly how did you perform them? exactly as its written in the manual protocol, by tapping the pale on absorbent paper.
thanks

Read More
Answer

Thank you for confirming those details.

I am not entirely sure why you are seeing such low readings. It is likely to do either with the standard itself or with the detection (antibody and HRP conjugate). What I would like to do if you wouldn't mind is provide you with a replacement kit free of charge to see if the kit has been damaged in any way. This is very uncommon but as I can see no problem in how you have been using the kit I would like to rule this out before trying some optimisation or the protocol.

If you would like for me to do this could you please provide me with the order number (or delivery address and approximate date of delivery) and I will have this arranged as soon as possible.

I look forward to receiving your reply.

Read More

Answer

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

In order to understand what may be contributing to the low readings and potentially offer solutionscould you please answer the following questions:

1. You mentioned thatyou used 3 strips. Were all of these using the standard provided with the kit? What results did you obtain with the other 2 strips?

2. Were all reagents allowed to come to room temperature before use?

3.Exactly how was the standard prepared and diluted? What diluent was used?

4. When washing, was the excess liquid removed by aspiration or tapping plate on absorbent paper?

5. Was the TMB solution protected from the light?

6. What colour was the TMB solution before adding to the wells?

7. How was the biotinylated anti-IL-6 prepared?

8. How was the Streptavidin-HRP prepared?

9. Were the strips covered during the 3 hour incubation of the biotinylated anti-IL6?

10. Was the HRP solution prepared directly prior to use?

11.At whatwavelength was the absorbancemeasured? What kind of reader have you been using?

12. The wash steps you refer to, after which steps werethese performed and exactly how did you perform them?

I am sorry for all the questions but I am hoping to be able to understand what may be contributing to the low readings you have been observing with the standard. If possible could you also provide me with the order number or the approximate date of delivery and the delivery address?

I thank you for your cooperation and I look forward to receiving your reply.

Read More

Question
Answer

Thanks for your enquiry.



Those kits are suitable for all body fluids, so urine is applicable.






I hope this helps.

Read More

Answer

Thank you for contacting us. I apologise for the delay.
Unfortunately any of these kits have been tested with human ascites. However, they should work as they are suitable for all body fluids.
The only problem I would suspect is the high concentration of IgG in ascites which can interfere with the ELISA. I would suggest diluting samples 1:2 or 1:5 to minimize the matrix effect.
I hope this helps and if we can assist further, please do not hesitate to contact us.

Read More

Answer

Thank you for your enquiry. I can confirm that both these kits ab46042 and ab108826 should be suitable for use with saliva samples. I can confirm that  kit ab108826 has been tested on saliva samples,  We suggest to use saliva samples at 1x (no dilution) for this kit. We would suggest ab46042 should be suitable for saliva samples. However, this has not been specifically tested. Therefore, we are able to offer a discount for this kit in return for agreement to submit an Abreview with the data. I am currently waiting to hear from my colleagues how much of a discount we can offer and will be in touch with you as soon as possible. I hope this will be helpful. If you have any further questions, please do let me know. I will be in touch again regarding ab46052.  

Read More

11-15 of 15 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up