Overview

  • Product name
    Human IL-6 ELISA Set (without plates)
    See all IL-6 kits
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 2 pg/ml
  • Range
    200 pg/ml - 6.25 pg/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-6 Human in vitro ELISA Set is designed for the quantitative measurement of IL-6 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.


     


    The IL-6 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.


     


    This ELISA set is intended to measure human IL-6 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.



    Sensitivity: < 2 pg / ml

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Reagents

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 5 x 96 tests 10 x 96 tests 15 x 96 tests
    Detection biotinylated anti-IL-6 antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml
    IL-6 Capture antibody 1 x 0.25ml 2 x 0.25ml 3 x 0.25ml
    IL-6 Standard (Lyophilized) Yellow 5 vials 10 vials 15 vials
    Ready-to-use TMB 2 vials 4 vials 6 vials
    Streptavidin-HRP 1 x 25µl 2 x 25µl 3 x 25µl
  • Research areas
  • Function
    Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • Involvement in disease
    Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Sequence similarities
    Belongs to the IL-6 superfamily.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • Interleukin BSF 2
    • B cell differentiation factor
    • B cell stimulatory factor 2
    • B-cell stimulatory factor 2
    • BSF 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • Hepatocyte stimulatory factor
    • HGF
    • HSF
    • Hybridoma growth factor
    • Hybridoma growth factor Interferon beta-2
    • IFN-beta-2
    • IFNB2
    • IL 6
    • IL-6
    • IL6
    • IL6_HUMAN
    • Interferon beta 2
    • Interferon beta-2
    • Interleukin 6
    • Interleukin 6 (interferon beta 2)
    • Interleukin BSF 2
    • Interleukin-6
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab48478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • IL-6 Standard curve ranging from 6.25 to 200 pg/mL

References

ab48478 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for your enquiry. I apologise for the delay in obtaining this information for you. We have been waiting for confirmation from our source.

I can confirm the reagents and materials required but not provided with ab48478 IL6 Human Elipair Kit are as follows:

- 96 wells polysterene plate e.g. Nunc Maxisorp
- Coating Buffer : PBS pH 7.2-7.4
- Wash Buffer : PBS containing 0.05% tween 20 v/v
- Saturation Buffer : PBS 5% BSA w/v
- Standard Diluent Buffer : PBS 1%BSA w/v
- Biotinylated Antibody Diluent Buffer : PBS 1%BSAw/v
- HRP-Streptavidin Diluent Buffer : PBS 0.1% tween 20 plus 1% BSA w/v
- PBS 0.1% Azide W/v
- 1M Sulfuric Acid
- Automatic plate washer (Optional)
- ELISA plate reader 450nm

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Answer

Thank you for contacting us. I apologise for the delay.
Unfortunately any of these kits have been tested with human ascites. However, they should work as they are suitable for all body fluids.
The only problem I would suspect is the high concentration of IgG in ascites which can interfere with the ELISA. I would suggest diluting samples 1:2 or 1:5 to minimize the matrix effect.
I hope this helps and if we can assist further, please do not hesitate to contact us.

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Answer

Thank you for contacting us.

1) Our Elipair kits only contain the reagents needed to perform the assay, i.e. they include both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step. Unfortunately, ELISA plates are not supplied.

2) n/a. If you require kits including plates I may recommend ab108865 and ab108867, respectively.

Click here (or use the following: https://www.abcam.com/index.html?datasheet=108865).
Click here (or use the following: https://www.abcam.com/index.html?datasheet=108867).

3) We can recommend any polystyrene 1x96 well or 12x8 well plate suitable for ELISA.

4) Please find attached the protocols for ab48478 and ab47351. The suggested procedures may need some optimization depending on the plastic, matrix, standard range.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your reply.

I can suggest the same would apply for ab48478. I can recommend to purchase ELISA plates specifically. to ensure they are of the higher quality required.

I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

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Answer

Thank you for contacting us. Overnight Incubation of plate at 4C is sort of general recommendation we provide to our customers. 3-4 hours at 4C can also be tried; the assay should be OK However in case of no readings we would be suggesting the overnight incubation also. The assay should read at 450nm wavelength which should be fine with standard filters. Any reference filter can be used. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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