• Product name

    Human IL-6 Receptor ELISA Kit
    See all IL-6R kits
  • Detection method

  • Precision

    Sample n Mean SD CV%
    Sample A 7 977.7pg/ml 3.04 0.31%
    Sample B 7 131.1pg/ml 2.489 2.289%
    Sample n Mean SD CV%
    Sample A 24 983.9pg/ml 10.46 1.06%
    Sample B 24 136.2pg/ml 6.086 4.46%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 5 pg/ml
  • Range

    31.25 pg/ml - 1000 pg/ml
  • Recovery

    96 %

  • Assay time

    1h 40m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s IL-6 Receptor (Interleukin-6 Receptor) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of soluble IL-6 Receptor in Human serum, plasma, buffered solutions and cell culture medium.

    A monoclonal antibody specific for IL-6 Receptor has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-6 Receptor concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-6 Receptor are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-6 Receptor present in the samples. 

    This kit will recognize both endogenous and recombinant Human IL-6 Receptor.

    Get higher sensitivity in only 90 minutes with Human IL-6 Receptor ELISA Kit (ab181424) from our SimpleStep ELISA® range.


  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform



  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml
    Biotinylated anti-IL-6R (CD126) Red 1 x 0.4ml 2 x 0.4ml
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IL-6 Receptor Microplate (12 x 8 well strips) 1 unit 2 units
    IL-6R Control Silver 2 vials 4 vials
    IL-6R Standard Yellow 2 vials 4 vials
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • Research areas

  • Function

    Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
    Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity.
  • Tissue specificity

    Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum.
  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 3 subfamily.
    Contains 1 fibronectin type-III domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • Domain

    The two fibronectin type-III-like domains, contained in the N-terminal part, form together a cytokine-binding domain.
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
  • Post-translational

    A short soluble form may also be released from the membrane by proteolysis.
  • Cellular localization

    Secreted and Basolateral cell membrane.
  • Information by UniProt
  • Alternative names

    • CD126
    • CD126 antigen
    • Gp80
    • IL 6R alpha
    • IL-6 receptor subunit alpha
    • IL-6R 1
    • IL-6R subunit alpha
    • IL-6R-1
    • IL-6R-alpha
    • IL-6RA
    • Il6r
    • IL6R1
    • IL6RA
    • IL6Ralpha
    • Interleukin 6 receptor
    • Interleukin-6 receptor subunit alpha
    • Membrane glycoprotein 80
    • MGC104991
    • OTTHUMP00000034320
    see all
  • Database links

Associated products


Our Abpromise guarantee covers the use of ab46029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative Standard Curve using ab46029



This product has been referenced in:

  • Hosseinkhani B  et al. Extracellular Vesicles Work as a Functional Inflammatory Mediator Between Vascular Endothelial Cells and Immune Cells. Front Immunol 9:1789 (2018). Read more (PubMed: 30131806) »
  • Osuala KO  et al. Il-6 signaling between ductal carcinoma in situ cells and carcinoma-associated fibroblasts mediates tumor cell growth and migration. BMC Cancer 15:584 (2015). Sandwich ELISA ; Human . Read more (PubMed: 26268945) »
See all 2 Publications for this product

Customer reviews and Q&As

Abreview for IL6R Human ELISA Kit

Good Good 4/5 (Ease of Use)

Sample: Human Cell (lung cancer cell line)
Specification: lung cancer cell line
Amount of Sample: about 1,000,000 cells µg
Type of Kit Used: ELISA
Positive control: The control vials in the kit (430.9 pg/mL)
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Optimized incubation time of TMB substrate solution was 10 minutes in our laboratory. With15 minutes Incubation, the O.D. value of 1000 pg/mL standard reached over 2.5,

Mrs. Hee Yi

Verified customer

Submitted May 07 2009

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