Overview

  • Product name

    Human IL-7 ELISA Kit
    See all IL-7 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    5% NHS 9 3%
    Inter-assay
    Sample n Mean SD CV%
    5% NHS 3 3%
  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.19 pg/ml
  • Range

    1.6 pg/ml - 100 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 95 84% - 107%
    Cell culture media 93 85% - 102%
    Hep Plasma 100 94% - 110%
    EDTA Plasma 106 102% - 120%
    Cit plasma 96 88% - 103%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    IL-7 (Interleukin-7) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-7 protein in human serum, plasma and cell culture supernatants.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:


    Samples diluted in Sample Diluent NS - 0.67 pg/mL
    Samples diluted in Sample Diluent 75BP - 0.24 pg/mL
    Samples diluted in Sample Diluent 25BP - 0.19 pg/mL

  • Notes

    Interleukin 7 (IL-7) is a 20kDa hematopoietic growth factor secreted by stromal cells residing in the bone marrow, thymus, spleen and gut. IL-7 plays a critical role during early T-cell development of naïve and memory CD8+ T cells, as well as during the proliferation and differentiation of B cells. Furthermore, IL-7 has been shown to play a key role in coreceptor reversal, a process which induces the expression of CD8 receptor in a T-cell that has begun differentiation as a CD4+ T cell.

    IL-7 belongs to a family of short-chain four-helical bundle cytokines. These family members signal through heterodimeric receptor complexes that contain the common cytokine receptor gamma-chain, and activate signaling pathways that promote cell proliferation and survival such as the PI3K, MAPK, and JAK/STAT.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human IL-7 Capture Antibody 1 x 600µl
    10X Human IL-7 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BI 1 x 6ml
    Human IL-7 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 75BP 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Relevance

    IL-7 is a cytokine important for B and T cell development. It and the hepatocyte growth factor form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. IL-7 is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. It can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that IL-7 plays an essential role in lymphoid cell survival.
  • Cellular localization

    Secreted
  • Alternative names

    • IL 7
    • IL7
    • IL7 nirs variant 6
    • Interleukin 7
    • Interleukin7
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab185984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Ten individual healthy donors were evaluated for the presence of IL-7 in serum using this assay. Results were interpolated from the standard curve in Sample Diluent 75BP and corrected by sample dilution (20 fold). Four individuals had levels of IL-7 above the limit of detection. The mean IL-7 is 13.5 pg/mL with a range of 6.6 – 21.8 pg/mL and a standard deviation of 6.2 pg/mL. Donors # 3-7 and 9 were below the limit of detection.

Protocols

References

This product has been referenced in:

See 1 Publication for this product

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