Human IL-8 ELISA Kit (ab100575)
Key features and details
- Sensitivity: 1 pg/ml
- Range: 0.8 pg/ml - 600 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-8 ELISA Kit
See all IL-8 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 1 pg/ml -
Range
0.8 pg/ml - 600 pg/ml -
Recovery
95 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 96.18 85% - 106% Serum 95.34 84% - 107% Plasma 93.85 83% - 104% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s IL-8 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-8 in serum, plasma, and cell culture supernates.
This assay employs an antibody specific for Human IL-8 coated on a 96-well plate. Standards and samples are pipette into the wells and IL-8 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL- 8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-8 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimization may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human IL-8 2 vials IL-8 Microplate (12 x 8 wells) 1 unit Recombinant Human IL-8 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsSeveral N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- (Ala-IL-8)77
- (Ser-IL-8)72
- 9E3
see all -
Database links
- Entrez Gene: 3576 Human
- Omim: 146930 Human
- SwissProt: P10145 Human
- Unigene: 624 Human
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab100575 has been referenced in 5 publications.
- Arkusz K & Paradowska E Impedimetric Detection of Femtomolar Levels of Interleukin6, Interleukin 8, and Tumor Necrosis Factor Alpha Based on Thermally Modified Nanotubular Titanium Dioxide Arrays. Nanomaterials (Basel) 10:N/A (2020). PubMed: 33266223
- Abd El Maksoud AI et al. Selective Regulation of B-Raf Dependent K-Ras/Mitogen-Activated Protein by Natural Occurring Multi-kinase Inhibitors in Cancer Cells. Front Oncol 9:1220 (2019). PubMed: 31781509
- Sahbudak Bal Z et al. Diagnostic Accuracy of Interleukin-6, Interleukin-8, and Interleukin-10 for Predicting Bacteremia in Children with Febrile Neutropenia. Turk J Haematol 34:254-257 (2017). PubMed: 28148470
- Giles KM et al. microRNA-7-5p inhibits melanoma cell proliferation and metastasis by suppressing RelA/NF-?B. Oncotarget 7:31663-80 (2016). PubMed: 27203220
- Giles KM et al. Axl mediates acquired resistance of head and neck cancer cells to the epidermal growth factor receptor inhibitor erlotinib. Mol Cancer Ther 12:2541-58 (2013). PubMed: 24026012