Key features and details
- Sensitivity: 1 pg/ml
- Range: 0.8 pg/ml - 600 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman IL-8 ELISA Kit
See all IL-8 kits
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 1 pg/ml
Range0.8 pg/ml - 600 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 96.18 85% - 106% Serum 95.34 84% - 107% Plasma 93.85 83% - 104%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s IL-8 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-8 in serum, plasma, and cell culture supernates.
This assay employs an antibody specific for Human IL-8 coated on a 96-well plate. Standards and samples are pipette into the wells and IL-8 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL- 8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-8 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human IL-8 (lyophilized) 2 vials IL-8 Microplate (12 x 8 wells) 1 unit Recombinant Human IL-8 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
FunctionIL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
modificationsSeveral N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
- Information by UniProt
- 3 10C
ab100575 has been referenced in 3 publications.
- Sahbudak Bal Z et al. Diagnostic Accuracy of Interleukin-6, Interleukin-8, and Interleukin-10 for Predicting Bacteremia in Children with Febrile Neutropenia. Turk J Haematol 34:254-257 (2017). PubMed: 28148470
- Giles KM et al. microRNA-7-5p inhibits melanoma cell proliferation and metastasis by suppressing RelA/NF-?B. Oncotarget 7:31663-80 (2016). PubMed: 27203220
- Giles KM et al. Axl mediates acquired resistance of head and neck cancer cells to the epidermal growth factor receptor inhibitor erlotinib. Mol Cancer Ther 12:2541-58 (2013). PubMed: 24026012