Human IL-8 ELISA Kit (ab108869)
- Datasheet
- References
- Protocols
Overview
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Product name
Human IL-8 ELISA Kit
See all IL-8 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.1% Inter-assay Sample n Mean SD CV% Overall 7.6% -
Sample type
Cell culture supernatant, Serum, Plasma, Tissue -
Assay type
Sandwich (quantitative) -
Sensitivity
> 0.01 ng/ml -
Range
0.016 ng/ml - 1 ng/ml -
Recovery
97 %
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Assay time
5h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s IL-8 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-8 concentrations in Human cell culture supernatants, tissue extracts, serum and plasma.
An IL-8 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-8 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-8 captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human IL-8 Antibody 1 x 120µl Chromogen Substrate 1 x 8ml IL-8 Microplate (12 x 8 well strips) 1 unit IL-8 Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsSeveral N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- (Ala-IL-8)77
- (Ser-IL-8)72
- Beta thromboglobulin like protein
see all -
Database links
- Entrez Gene: 3576 Human
- SwissProt: P10145 Human
- Unigene: 624 Human
Images
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Typical Standard CurveRepresentative Standard Curve using ab108869.
Protocols
References
ab108869 has not yet been referenced specifically in any publications.
