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    human-il-8-elisa-kit-ab108869.pdf

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Cardiovascular Angiogenesis Cytokines Interleukin
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Human IL-8 ELISA Kit (ab108869)

  • Datasheet
  • SDS
  • Protocol Booklet
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Typical Standard Curve

    Key features and details

    • Sensitivity: 0.01 ng/ml
    • Range: 0.016 ng/ml - 1 ng/ml
    • Sample type: Cell culture supernatant, Plasma, Serum, Tissue
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

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    Overview

    • Product name

      Human IL-8 ELISA Kit
      See all IL-8 kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall 4.1%
      Inter-assay
      Sample n Mean SD CV%
      Overall 7.6%
    • Sample type

      Cell culture supernatant, Serum, Plasma, Tissue
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      > 0.01 ng/ml
    • Range

      0.016 ng/ml - 1 ng/ml
    • Recovery

      97 %

    • Assay time

      5h 00m
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      Abcam’s IL-8 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-8 concentrations in Human cell culture supernatants, tissue extracts, serum and plasma.


      An IL-8 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-8 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-8 captured in plate.


      The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 1 x 96 tests
      100X Streptavidin-Peroxidase Conjugate 1 x 80µl
      10X Diluent N Concentrate 1 x 30ml
      20X Wash Buffer Concentrate 2 x 30ml
      50X Biotinylated Human IL-8 Antibody 1 x 120µl
      Chromogen Substrate 1 x 8ml
      IL-8 Microplate (12 x 8 well strips) 1 unit
      IL-8 Standard 1 vial
      Sealing Tapes 3 units
      Stop Solution 1 x 12ml
    • Research areas

      • Cardiovascular
      • Angiogenesis
      • Cytokines
      • Interleukin
      • Immunology
      • Innate Immunity
      • Cytokines
      • Interleukins
      • Immunology
      • Innate Immunity
      • Cytokines
      • Other
      • Cancer
      • Invasion/microenvironment
      • Angiogenesis
      • Angiogenic growth factors
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Interleukin ELISA kits
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Cytokines and cytokine receptors ELISA kits
    • Function

      IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
    • Sequence similarities

      Belongs to the intercrine alpha (chemokine CxC) family.
    • Post-translational
      modifications

      Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P10145 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • (Ala-IL-8)77
      • (Ser-IL-8)72
      • 9E3
      • Beta thromboglobulin like protein
      • C-X-C motif chemokine 8
      • CEF-4
      • chemokine, CXC motif, ligand 8
      • CXCL8
      • Emoctakin
      • GCP-1
      • GCP/IL-8 protein I
      • GCP/IL-8 protein II
      • GCP/IL-8 protein III
      • GCP/IL-8 protein IV
      • GCP/IL-8 protein V
      • GCP/IL-8 protein VI
      • GCP1
      • Granulocyte chemotactic protein 1
      • IL-8
      • IL-8(1-77)
      • IL-8(9-77)
      • IL8
      • IL8/NAP1 form I
      • IL8/NAP1 form II
      • IL8/NAP1 form III
      • IL8/NAP1 form IV
      • IL8/NAP1 form V
      • IL8/NAP1 form VI
      • IL8_HUMAN
      • Inteleukin 8
      • LECT
      • LUCT
      • Lymphocyte-derived neutrophil-activating factor
      • LYNAP
      • MDNCF
      • MDNCF-b
      • MDNCF-c
      • MONAP
      • Monocyte derived neutrophil activating peptide
      • Monocyte derived neutrophil chemotactic factor
      • Monocyte-derived neutrophil chemotactic factor
      • Monocyte-derived neutrophil-activating peptide
      • NAF
      • NAP 1
      • NAP-1
      • NAP1
      • Neutrophil activating peptide 1
      • Neutrophil activating protein 1
      • Neutrophil-activating factor
      • Neutrophil-activating protein 1
      • Protein 3 10C
      • Protein 3-10C
      • SCYB 8
      • SCYB8
      • Small inducible cytokine subfamily B member 8
      • T cell chemotactic factor
      • T-cell chemotactic factor
      • TSG 1
      • TSG1
      see all
    • Database links

      • Entrez Gene: 3576 Human
      • Omim: 146930 Human
      • SwissProt: P10145 Human
      • Unigene: 624 Human

      Associated products

        Images

        • Typical Standard Curve
          Typical Standard Curve

          Representative Standard Curve using ab108869.

        Protocols

        • Protocol Booklet

        Click here to view the general protocols

        Datasheets and documents

        • Datasheet
        • SDS
      • References (0)

        Publishing research using ab108869? Please let us know so that we can cite the reference in this datasheet.

        ab108869 has not yet been referenced specifically in any publications.

        Customer reviews and Q&As

        Show All Reviews Q&A
        Submit a review Submit a question

        Question

        Dear Tech Support Team,

        Kindly advise if the following products can be used with human ascites:

        https://www.abcam.com/High-Sensitivity-IL6-Human-ELISA-Kit-1-x-96-Well-Plate-ab46042.html

        https://www.abcam.com/High-Sensitivity-IL6-Human-ELISA-Kit-2-x-96-Well-Plates-ab46044.html

        https://www.abcam.com/IL6-Human-Elipair-Kit-Reagents-for-10-x-96-Well-Plates-ab48479.html

        https://www.abcam.com/IL6-Human-Elipair-Kit-Reagents-for-5-x-96-Well-Plates-ab48478.html

        https://www.abcam.com/IL6-Human-ELISA-Kit-ab108867.html

        https://www.abcam.com/IL6-Human-ELISA-Kit-1-x-96-Well-Plate-ab46027.html

        https://www.abcam.com/IL8-Human-ELISA-Kit-1-x-96-Well-Plate-ab46032.html

        https://www.abcam.com/IL8-Human-ELISA-Kit-ab100575.html

        https://www.abcam.com/IL8-Human-ELISA-Kit-ab108869.html

        https://www.abcam.com/IL8-Human-ELISA-Kit-2-x-96-Well-Plates-ab46049.html

        https://www.abcam.com/IL8-Human-ELISA-Kit-1-x-96-Well-Plate-ab100576.html

        Thanks in advance for your assistance and reply.
        Best Regards,

        Read More

        Abcam community

        Verified customer

        Asked on Feb 27 2012

        Answer

        Thank you for contacting us. I apologise for the delay.
        Unfortunately any of these kits have been tested with human ascites. However, they should work as they are suitable for all body fluids.
        The only problem I would suspect is the high concentration of IgG in ascites which can interfere with the ELISA. I would suggest diluting samples 1:2 or 1:5 to minimize the matrix effect.
        I hope this helps and if we can assist further, please do not hesitate to contact us.

        Read More

        Abcam Scientific Support

        Answered on Feb 27 2012

        Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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