We measured the concentration of human plasma IL-8 by using this Human IL-8 ELISA Kit (ab214030). Blood were collected in the vacutainer EDTA tubes, the upper layer of plasma was collected after centrifugation and frozen until determination. Samples were further diluted at 1:4 into Sample Diluent assay according to the protocol, the final optical density from all of samples were between the lowest and the highest standard. y-axis: O.D.
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Submitted Apr 14 2022