• Product name

    Human IL-8 ELISA Kit
    See all IL-8 kits
  • Detection method

  • Precision

    Sample n Mean SD CV%
    Pooled Data 3.1%
    Sample n Mean SD CV%
    Sample 1 18 1682pg/ml 152 9%
    Sample 2 18 1004pg/ml 111 11%
    Sample 3 18 768pg/ml 69 8.9%
    Sample 4 18 1497pg/ml 149 9.9%
    Sample 5 18 880pg/ml 83 9.4%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 25 pg/ml
  • Range

    62.5 pg/ml - 2000 pg/ml
  • Recovery

    88 %

  • Assay time

    1h 40m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human IL-8 ELISA kit is designed for the quantitative measurement of IL-8 supernatants, buffered solutions, serum and plasma samples. (Genecards: CXCL8)

    A monoclonal antibody specific for IL-8 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-8 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-8 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-8 present in the samples.

    This kit will recognize both endogenous and recombinant Human IL-8.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform



  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Biotinylated anti-IL-8 Red 1 x 0.4ml 2 x 0.4ml
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IL-8 Control Silver 2 vials 4 vials
    IL-8 Microplate (12 x 8 well strips) 1 unit 2 units
    IL-8 Standard Yellow 2 vials 4 vials
    Plastic Plate Covers 2 units 4 units
    Standard Diluent (Serum) 1 x 7ml 2 x 7ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • Research areas

  • Function

    IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational

    Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
  • Cellular localization

  • Information by UniProt
  • Alternative names

    • (Ala-IL-8)77
    • (Ser-IL-8)72
    • 3 10C
    • 3-10C
    • AMCF I
    • AMCF-I
    • AMCFI
    • b ENAP
    • B-ENAP
    • Beta thromboglobulin like protein
    • Beta-thromboglobulin-like protein
    • C X C motif chemokine 8
    • C-X-C motif chemokine 8
    • Chemokine (C X C motif) ligand 8
    • CXCL8
    • Emoctakin
    • GCP 1
    • GCP-1
    • GCP/IL-8 protein I
    • GCP/IL-8 protein II
    • GCP/IL-8 protein III
    • GCP/IL-8 protein IV
    • GCP/IL-8 protein V
    • GCP/IL-8 protein VI
    • GCP1
    • Granulocyte chemotactic protein 1
    • IL 8
    • IL-8
    • IL-8(1-77)
    • IL-8(9-77)
    • IL8
    • IL8/NAP1 form I
    • IL8/NAP1 form II
    • IL8/NAP1 form III
    • IL8/NAP1 form IV
    • IL8/NAP1 form V
    • IL8/NAP1 form VI
    • IL8_HUMAN
    • Interleukin 8
    • K60
    • LECT
    • LUCT
    • Lymphocyte derived neutrophil activating factor
    • Lymphocyte-derived neutrophil-activating factor
    • LYNAP
    • MDNCF
    • MDNCF-b
    • MDNCF-c
    • MONAP
    • Monocyte derived neutrophil activating peptide
    • Monocyte derived neutrophil chemotactic factor
    • Monocyte-derived neutrophil chemotactic factor
    • Monocyte-derived neutrophil-activating peptide
    • NAF
    • NAP 1
    • NAP-1
    • NAP1
    • Neutrophil activating peptide 1
    • Neutrophil activating protein 1
    • Neutrophil-activating factor
    • Neutrophil-activating protein 1
    • Protein 3 10C
    • Protein 3-10C
    • SCYB8
    • Small inducible cytokine subfamily B member 8
    • T cell chemotactic factor
    • T-cell chemotactic factor
    • TSG 1
    • TSG-1
    • TSG1
    see all
  • Database links


Our Abpromise guarantee covers the use of ab46032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative Standard Curve using ab46032

  • IL-8 detected in supernatants from control cells (C) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) (P), and with the addition of 1 ug x mL-1 of LPS (Sigma) (P/L) for the last 6 hours. The positive control is included (Pos.). Results shown after background signal was subtracted (duplicates +/- SD).



This product has been referenced in:

  • Quan B  et al. miR-141 alleviates LPS-induced inflammation injury in WI-38 fibroblasts by up-regulation of NOX2. Life Sci 216:271-278 (2019). Read more (PubMed: 30500550) »
  • Ishii N  et al. Conophylline suppresses pancreatic cancer desmoplasia and cancer-promoting cytokines produced by cancer-associated fibroblasts. Cancer Sci 110:334-344 (2019). Read more (PubMed: 30353606) »
See all 24 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Abreview for IL-8 ELISA Kit

Good Excellent 5/5 (Ease of Use)
This kit was used to measure the level of IL-8 secretion in to the medium after infecting cells with E.coli strains. The kit was nicely sensitive to detect the level of IL-8 in the medium and was very helpful.
The standards and the control vial worked very nicely and gave a nice curve.

Abcam user community

Verified customer

Submitted Aug 13 2019


Nous ne recommandons pas de modifier les concentrations des courbes standards mais plutôt de diluer vos échantillons.

Toutefois, si vous voulez vraiment changer les courbes standards ceci est possible. Cependant en augmentant les concentrations de la courbe standard, vous augmentez considérablement le risque de saturer l'anticorps qui peut conduire à un effet de plafonnement (une courbe non linéaire). N’oublions pas que l’anticorps de capture a aussi une limite du taux de protéine qu’il peut capturer.

Veuillez noter que beaucoup de tests ont été réalisé pour déterminer les concentrations standards optimales pour les anticorps et donc si vous décidez de modifier les gammes de standards nous ne pourrons plus garantir que ces produits fonctionneront.

De plus, nous ne savons pas à partir de quelle concentration les standards sont solubles.

Pour ab100603, nous recommandons de reconstituer le standard dans 400 ul de diluant pour faire 100 ng/ml. Ceci est après dilué ˜5.555 fois pour faire une concentration de 18,000 pg/ml. Je vous conseille donc de diluer le standard de 100 ng/ml moins que 5.55 fois pour obtenir une gamme plus concentré.

Pour ab46032, nous ne recommandons pas de reconstituer le standard dans un plus petit volume, pour obtenir 4.000 pg/mL.

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Thank you very much for your call yesterday and for your patience while I have been in touch with the lab about ab46032.

This kit is suitable for all body fluids. Urine samples should be diluted in standard diluent buffer (containing PBS BSA). We have no data to report on urine samples, as the titers are variable, but we suggest starting the dilution at 1:1 (50 uL of samples and 50 uL of diluent).

I hope that this information will be useful, but please let me know if you have any further questions and I'll be happy to help.

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Thank you for contacting us. I apologise for the delay.
Unfortunately any of these kits have been tested with human ascites. However, they should work as they are suitable for all body fluids.
The only problem I would suspect is the high concentration of IgG in ascites which can interfere with the ELISA. I would suggest diluting samples 1:2 or 1:5 to minimize the matrix effect.
I hope this helps and if we can assist further, please do not hesitate to contact us.

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Thank you for your phone call yesterday and for your questions about our ELISA kits. I have heard back from the lab regarding ab46032, and unfortunately, the kit was never assayed on murine samples. So, I would not recommend this kit to use with mouse samples, but you may be able to find a mouse IL8 kit from another vendor by searching on www.biocompare.com. Please let me know if you have any questions!

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Thank you for your enquiry. There's no Thimerosal in any component of the kit. However, we use azide (0.1%) in standard diluent and Biotinylated antibody diluent, and Proclin (0.1%) in HRP diluent.

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