Overview

  • Product name

    Human IL-8 ELISA Kit
    See all IL-8 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Pooled Data 3.1%
    Inter-assay
    Sample n Mean SD CV%
    Sample 1 18 1682pg/ml 152 9%
    Sample 2 18 1004pg/ml 111 11%
    Sample 3 18 768pg/ml 69 8.9%
    Sample 4 18 1497pg/ml 149 9.9%
    Sample 5 18 880pg/ml 83 9.4%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 25 pg/ml
  • Range

    62.5 pg/ml - 2000 pg/ml
  • Recovery

    88 %

  • Assay time

    1h 40m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human IL-8 ELISA kit is designed for the quantitative measurement of IL-8 supernatants, buffered solutions, serum and plasma samples. (Genecards: CXCL8)


    A monoclonal antibody specific for IL-8 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-8 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-8 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-8 present in the samples.


    This kit will recognize both endogenous and recombinant Human IL-8.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Biotinylated anti-IL-8 Red 1 x 0.4ml 2 x 0.4ml
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    IL-8 Control Silver 2 vials 4 vials
    IL-8 Microplate (12 x 8 well strips) 1 unit 2 units
    IL-8 Standard Yellow 2 vials 4 vials
    Plastic Plate Covers 2 units 4 units
    Standard Diluent (Serum) 1 x 7ml 2 x 7ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • Research areas

  • Function

    IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications

    Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • (Ala-IL-8)77
    • (Ser-IL-8)72
    • 3 10C
    • 3-10C
    • AMCF I
    • AMCF-I
    • AMCFI
    • b ENAP
    • B-ENAP
    • Beta thromboglobulin like protein
    • Beta-thromboglobulin-like protein
    • C X C motif chemokine 8
    • C-X-C motif chemokine 8
    • Chemokine (C X C motif) ligand 8
    • CXCL8
    • Emoctakin
    • GCP 1
    • GCP-1
    • GCP/IL-8 protein I
    • GCP/IL-8 protein II
    • GCP/IL-8 protein III
    • GCP/IL-8 protein IV
    • GCP/IL-8 protein V
    • GCP/IL-8 protein VI
    • GCP1
    • Granulocyte chemotactic protein 1
    • IL 8
    • IL-8
    • IL-8(1-77)
    • IL-8(9-77)
    • IL8
    • IL8/NAP1 form I
    • IL8/NAP1 form II
    • IL8/NAP1 form III
    • IL8/NAP1 form IV
    • IL8/NAP1 form V
    • IL8/NAP1 form VI
    • IL8_HUMAN
    • Interleukin 8
    • K60
    • LECT
    • LUCT
    • Lymphocyte derived neutrophil activating factor
    • Lymphocyte-derived neutrophil-activating factor
    • LYNAP
    • MDNCF
    • MDNCF-b
    • MDNCF-c
    • MONAP
    • Monocyte derived neutrophil activating peptide
    • Monocyte derived neutrophil chemotactic factor
    • Monocyte-derived neutrophil chemotactic factor
    • Monocyte-derived neutrophil-activating peptide
    • NAF
    • NAP 1
    • NAP-1
    • NAP1
    • Neutrophil activating peptide 1
    • Neutrophil activating protein 1
    • Neutrophil-activating factor
    • Neutrophil-activating protein 1
    • Protein 3 10C
    • Protein 3-10C
    • SCYB8
    • Small inducible cytokine subfamily B member 8
    • T cell chemotactic factor
    • T-cell chemotactic factor
    • TSG 1
    • TSG-1
    • TSG1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab46032

  • IL-8 detected in supernatants from control cells (C) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) (P), and with the addition of 1 ug x mL-1 of LPS (Sigma) (P/L) for the last 6 hours. The positive control is included (Pos.). Results shown after background signal was subtracted (duplicates +/- SD).

Protocols

References

This product has been referenced in:

  • Quan B  et al. miR-141 alleviates LPS-induced inflammation injury in WI-38 fibroblasts by up-regulation of NOX2. Life Sci 216:271-278 (2019). Read more (PubMed: 30500550) »
  • Ishii N  et al. Conophylline suppresses pancreatic cancer desmoplasia and cancer-promoting cytokines produced by cancer-associated fibroblasts. Cancer Sci 110:334-344 (2019). Read more (PubMed: 30353606) »
See all 24 Publications for this product

Customer reviews and Q&As

Filter by Ratings

Abreview for IL-8 ELISA Kit

Good Excellent 5/5 (Ease of Use)
Abreviews
This kit was used to measure the level of IL-8 secretion in to the medium after infecting cells with E.coli strains. The kit was nicely sensitive to detect the level of IL-8 in the medium and was very helpful.
The standards and the control vial worked very nicely and gave a nice curve.

Abcam user community

Verified customer

Submitted Aug 13 2019

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