Human IL-8 ELISA Set (without plates) (ab48481)

Submit a review Q&A (1)References (2)

Overview

  • Product name
    Human IL-8 ELISA Set (without plates)
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 25 pg/ml
  • Range
    2000 pg/ml - 62.5 pg/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s IL-8 Human in vitro ELISA Set is designed for the quantitative measurement of IL-8 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.


    The IL-8 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.


    This ELISA set is intended to measure human IL-8 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.


    Sensitivity: < 29 pg / ml

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Reagents

Properties

Applications

Our Abpromise guarantee covers the use of ab48481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human IL-8 ELISA Set
    Human IL-8 ELISA Set
    IL-8 Standard curve ranging from 62.5 to 2000 pg/mL

References

This product has been referenced in:
  • Mollarasouli F  et al. Ultrasensitive determination of receptor tyrosine kinase with a label-free electrochemical immunosensor using graphene quantum dots-modified screen-printed electrodes. Anal Chim Acta 1011:28-34 (2018). Read more (PubMed: 29475482) »
  • Liang CL  et al. Protection Effect of Zhen-Wu-Tang on Adriamycin-Induced Nephrotic Syndrome via Inhibiting Oxidative Lesions and Inflammation Damage. Evid Based Complement Alternat Med 2014:131604 (2014). ELISA . Read more (PubMed: 24812565) »
See all 2 Publications for this product

Publishing research using ab48481? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Dear technical team,   Our customer purchased ab48481, ab47443 and ab48478.   She had experience using other bander's ELISA kit but ELIPAIR has different protocol that it needs 24hr drying time when coating the plate.   She thinks 2~3 hour is enough and wants to try short time drying so, please let her know why it needs 24 hour time to dry.   Second question is about reference filter. Is it necessary or optional?   "the absorbance at 630nm or 650nm" is different from other kit what she used before.   540~570nm are general absorbance for reference filter?   Please explain about this for our customer.   Best regards,  

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Answer

Thank you for contacting us. Overnight Incubation of plate at 4C is sort of general recommendation we provide to our customers. 3-4 hours at 4C can also be tried; the assay should be OK However in case of no readings we would be suggesting the overnight incubation also. The assay should read at 450nm wavelength which should be fine with standard filters. Any reference filter can be used. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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