Recombinant

Human IL-8 Matched Antibody Pair Kit (ab215402)

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Human IL-8 standard curve.
  • Sandwich ELISA - Human IL-8 Matched Antibody Pair Kit (ab215402)

Key features and details

  • Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
  • For economical ELISA and ELISA-based assay development
  • Reacts with: Human
  • Range: 1.9 pg/ml - 125 pg/ml

Overview

  • Product name

    Human IL-8 Matched Antibody Pair Kit
    See all IL-8 kits

  • Detection method

    Colorimetric

  • Assay type

    ELISA set

  • Sensitivity

    0.16 pg/ml

  • Range

    1.9 pg/ml - 125 pg/ml

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human IL-8 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human IL-8.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab214030. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.

  • Tested applications

    Suitable for: IA, ELISAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 5 x 96 tests
    Human IL-8 Capture Antibody 1 x 50µg
    Human IL-8 Detector Antibody 1 x 12.5µg
    Human IL-8 Lyophilized Protein 1 vial

  • Research areas

  • Function

    IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.

  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.

  • Post-translational
    modifications

    Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • (Ala-IL-8)77
    • (Ser-IL-8)72
    • 9E3
    • Beta thromboglobulin like protein
    • C-X-C motif chemokine 8
    • CEF-4
    • chemokine, CXC motif, ligand 8
    • CXCL8
    • Emoctakin
    • GCP-1
    • GCP/IL-8 protein I
    • GCP/IL-8 protein II
    • GCP/IL-8 protein III
    • GCP/IL-8 protein IV
    • GCP/IL-8 protein V
    • GCP/IL-8 protein VI
    • GCP1
    • Granulocyte chemotactic protein 1
    • IL-8
    • IL-8(1-77)
    • IL-8(9-77)
    • IL8
    • IL8/NAP1 form I
    • IL8/NAP1 form II
    • IL8/NAP1 form III
    • IL8/NAP1 form IV
    • IL8/NAP1 form V
    • IL8/NAP1 form VI
    • IL8_HUMAN
    • Inteleukin 8
    • LECT
    • LUCT
    • Lymphocyte-derived neutrophil-activating factor
    • LYNAP
    • MDNCF
    • MDNCF-b
    • MDNCF-c
    • MONAP
    • Monocyte derived neutrophil activating peptide
    • Monocyte derived neutrophil chemotactic factor
    • Monocyte-derived neutrophil chemotactic factor
    • Monocyte-derived neutrophil-activating peptide
    • NAF
    • NAP 1
    • NAP-1
    • NAP1
    • Neutrophil activating peptide 1
    • Neutrophil activating protein 1
    • Neutrophil-activating factor
    • Neutrophil-activating protein 1
    • Protein 3 10C
    • Protein 3-10C
    • SCYB 8
    • SCYB8
    • Small inducible cytokine subfamily B member 8
    • T cell chemotactic factor
    • T-cell chemotactic factor
    • TSG 1
    • TSG1
    see all

  • Database links

    Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab215402 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IA
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent concentration.
    Notes
    IA
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent concentration.

    Images

    • Human IL-8 standard curve.
      Human IL-8 standard curve.

      Standard calibration curve. Background subtracted values are graphed.

    • Sandwich ELISA - Human IL-8 Matched Antibody Pair Kit (ab215402)
      Sandwich ELISA - Human IL-8 Matched Antibody Pair Kit (ab215402)
      To learn more about the advantages of recombinant antibodies see here.

    References (0)

    Publishing research using ab215402? Please let us know so that we can cite the reference in this datasheet.

    ab215402 has not yet been referenced specifically in any publications.

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