Human IL-9 ELISA Kit (ab242237)
- Datasheet
- References
- Protocols
Overview
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Product name
Human IL-9 ELISA Kit
See all IL-9 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% supernatant 5 6.3% Inter-assay Sample n Mean SD CV% supernatant 3 6.5% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.3 pg/ml -
Range
9.38 pg/ml - 600 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 110 96% - 119% Serum 100 92% - 111% Hep Plasma 101 99% - 105% EDTA Plasma 102 97% - 111% Cit plasma 101 95% - 109% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
IL-9 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-9 protein in human serum, plasma, and cell culture supernatant samples.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
IL-9 is a cytokine produced by CD4+ T cells and is involved in hematopoietic cell regulation. IL-9 has been identified as a potential target for melanoma and asthma.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IL-9 Capture Antibody 1 x 600µl 10X Human IL-9 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human IL-9 Lyophilized recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 75BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Supports IL-2 independent and IL-4 independent growth of helper T-cells. -
Sequence similarities
Belongs to the IL-7/IL-9 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Cytokine P40
- Homolog of mouse T cell and mast cell growth factor 40
- HP40
see all -
Database links
- Entrez Gene: 3578 Human
- SwissProt: P15248 Human
- Unigene: 960 Human
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab242237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Other - Human IL-9 ELISA Kit (ab242237)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human IL-9 Standard Curve Comparison.Standard Curve comparison between human IL-9 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity. -
Example of human IL-9 standard curve in Sample Diluent 75BS.The IL-9 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native IL-9 in human HDLM-2 cell culture supernatant samples.The concentrations of IL-9 were measured in duplicates, interpolated from the IL-9 standard curves and corrected for sample dilution. Undiluted samples are as follows: HDLM-2 supernatant 1: 100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-9 concentration was determined to be 65.7 ng/ml in HDLM-2 supernatant.
Protocols
References
ab242237 has not yet been referenced specifically in any publications.
