• Product name
    Human IL10 + IL2 FLUOROSPOT Kit
    See all IL10 + IL2 kits
  • Detection method
  • Sample type
    Cell culture supernatant, Cell culture extracts
  • Assay type
    Sandwich (qualitative)
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, oncology, infectious diseases, autoimmune diseases and transplantation.

    This Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

    Principle of Method

    After cell stimulation, locally produced cytokines are captured by IL-10 and IL-2 specific monoclonal antibodies. After cell lysis, trapped cytokine molecules are revealed by a secondary anti- IL-10 FITC conjugated antibody and a biotinylated anti-IL-2 antibody. Those are in turn recognised by anti-FITC green fluorescent dye and streptavidin-phycoerythrin conjugates. PVDF-bottomed-well plates are then read under a UV light beam. Green fluorescent spots indicate IL-10 production while IL-2 is revealed by red spots. Yellow spots will indicate dual cytokine producing cells.

  • Tested applications
    Suitable for: FluroSpotmore details
  • Platform


  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 5 x 96 tests 10 x 96 tests
    96 PVDF-bottomed-well plates. 5 units 10 units
    Anti-FITC antibody green fluorescence conjugate. 1 vial 2 vials
    Biotinylated detection antibody for IL-2 (resuspend in 0.55mL). 1 vial 2 vials
    Bovine Serum Albumin 1 x 1g 2 x 1g
    Human IL-10 Capture antibody 1 x 500µl 2 x 500µl
    Capture antibody for IL-2 1 x 500µl 2 x 500µl
    FITC conjugated detection antibody for IL-10(resuspend in 0.55mL). 1 vial 2 vials
    Fluorescence buffer 1 x 2.5ml 2 x 2.5ml
    Streptavidin-phycoerythrin conjugate. 1 vial 2 vials
  • Research areas
  • Relevance
    IL2 is produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. IL10 inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • Cellular localization
  • Alternative names
    • Aldesleukin
    • CSIF
    • Cytokine synthesis inhibitory factor
    • Interleukin 10
    • Interleukin 2
    • Lymphokine
    • T cell growth factor
    • TCGF
    • TGIF
    see all
  • Database links


Our Abpromise guarantee covers the use of ab48460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FluroSpot Use at an assay dependent dilution.


ab48460 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Für ist eine Detektion diese Fluorospot Kits ist UV Licht zwingend notwendig. Dies kann mit einem UV-Licht Mikroskop für die Auszählung der Punkte per Hand geschehen oder auch mit einem ELISPOT Reader, der eine UV Lampe besitzt (z.B. von CTL, Zeiss ode...

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