Human IL17A + IL4 FLUOROSPOT Kit (without plates) (ab105259)


  • Product name
    Human IL17A + IL4 FLUOROSPOT Kit (without plates)
  • Detection method
  • Sample type
    Cell culture supernatant, Cell culture extracts
  • Assay type
    Sandwich (qualitative)
  • Assay duration
    Multiple steps standard assay
  • Product overview

    ab105259 is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in vitro manipulations allowing cytokine production analysis as close as possible to in vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Fluorospot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, oncology, infectious diseases, autoimmune diseases and transplantation.

    This Kit is based on sandwich immune-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

    After cell stimulation, locally produced cytokines are captured by IL17A and IL4 specific monoclonal antibodies. After cell lysis, trapped cytokine molecules are revealed by a secondary anti- Il17A FITC conjugated antibody and a biotinylated anti- IL4 antibody. Those are in turn recognised by anti-FITC green fluorescent dye and streptavidin-phycoerythrin conjugates. PVDF-bottomed-well plates are then read under a UV light beam. Green fluorescent spots indicate IL17A production while IL4 is revealed by red spots. Yellow spots will indicate dual cytokine producing cells.

    See Figure 1 for visual scheme.



  • Notes
    • Store kit reagents at 4°C except Plate which should be stored at room temperature.
    • If not used within a short period of time, reconstituted Detection Antibodies should be aliquoted and stored at -20°C. In these conditions the reagent is stable for at least one year.
    •  For optimal performance prepare Anti FITC-green fluorescence conjugate / Streptavidin-phycoerythrin immediately prior to use.




  • Tested applications
    Suitable for: FluroSpotmore details
  • Platform


  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests 10 x 96 tests
    Anti-FITC antibody green fluorescence conjugate. 1 vial 2 vials
    Biotinylated detection antibody for IL4 1 vial 2 vials
    Bovine Serum Albumin 1 x 1g 2 x 1g
    Capture antibody for IL17A 1 x 500µl 2 x 500µl
    Capture antibody for IL4 1 x 500µl 2 x 500µl
    FITC-conjugated detection antibody for IL17A (resuspend in 0.55mL) 1 vial 2 vials
    Fluorescence buffer 1 x 2.5ml 2 x 2.5ml
    Streptavidin-phycoerythrin conjugate. 1 vial 2 vials
  • Research areas
  • Relevance
    IL4; Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. IL17A: Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.
  • Cellular localization
  • Alternative names
    • B cell stimulatory factor 1
    • Binetrakin
    • BSF1
    • CTLA8
    • Cytotoxic T lymphocyte associated antigen 8
    • IL17
    • Interleukin 17A
    • Interleukin 4
    • Lymphocyte stimulatory factor 1
    • Pitrakinra
    see all


Our Abpromise guarantee covers the use of ab105259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FluroSpot Use at an assay dependent dilution.


  • Figure 1: Procedure Summary


ab105259 has not yet been referenced specifically in any publications.

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