Human Inflammation Antibody Array - Membrane (20 targets) (ab169804)

Overview

  • Product name
    Human Inflammation Antibody Array - Membrane (20 targets)
    See all Inflammation Antibody Array antibody arrays
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type
    Semi-quantitative
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab169804 detects 20 Human Inflammatory Factors in 4 or 8 samples. Suitable for all liquid sample types.


    Targets: Eotaxin-1, Eotaxin-2, G-CSF, GM-CSF, IFN-gamma, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, IL-10, IL-11, IL-12 p40, IL-12 p70, IL-13, I-309, TIMP-2


    Learn more about membrane antibody arrays

  • Notes

    If you are interested in this cytokine array, arrays ab133996ab133997ab169817ab133998 and ab134003 may also be of interest. A list of human antibody membrane arrays is available here.

  • Tested applications
    Suitable for: Multiplex Protein Detectionmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab169804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection Use at an assay dependent concentration.

Images

  • Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate.

    Cells were cultured unstimulated or stimulated with 10 mg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169804. Media alone was used as a negative control.

  • Cells were cultured unstimulated or stimulated with 10 mg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169804. Media alone was used as a negative control. Mean pixel density was quantified using CCD camera software analysis.

  • HepG2 cells (Human hepatocellular carcinoma) were seeded at 1x106 cells/mL and cultured in EMEM media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169804.

  • Conditioned media was harvested after 48 hours, aliquoted and assayed using ab169804. Mean pixel density was quantified using CCD camera software analysis.

  • Typical results obtained with Abcam Membrane Antibody Arrays. Image above presents typical images obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

Protocols

References

ab169804 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

The sensitivity level for our human inflammation antibody arrays was standardized as pg/ml sensitivity based on 4 pg/ml MCP-1 sensitivity, one of the targets in these arrays.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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