Human Inflammation Antibody Array - Membrane (40 Targets) (ab134003)

Overview

  • Product name
    Human Inflammation Antibody Array - Membrane (40 Targets)
    See all Inflammation Antibody Array antibody arrays
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type
    Semi-quantitative
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab134003 is for simultaneous detection of 40 Human Inflammatory Factors. Suitable for all sample types.


    Targets: Eotaxin, Eotaxin-2, GCSF, GM-CSF, ICAM-1, IFN-gamma, I-309, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-6, IL-6sR, IL-7, IL-8, IL-10, IL-11, IL-12p40, IL-12p70, IL-13, IL-15, IL-16, IL-17, IP-10, MCP-1, MCP-2, M-CSF, MIG, MIP-1alpha, MIP-1beta, MIP-1delta, RANTES, TGF-beta1, TNF-alpha, TNF-beta, sTNF RI, sTNF-RII, PDGF-BB, TIMP-2


    Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


    Learn more about membrane antibody arrays

  • Notes

    If you are interested in this cytokine array, arrays ab133997ab169817ab133998ab169804ab169805 and ab133996 may also be of interest.


    A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.

  • Tested applications
    Suitable for: Multiplex Protein Detectionmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab134003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection Use at an assay dependent concentration.

Images

  • Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 mg/mL PHA.

    Conditioned media was harvested after 48 hours, aliquoted and assayed using ab134003. Media alone was used as a negative control.

  • Conditioned media was harvested after 48 hours, aliquoted and assayed using ab134003. Media alone was used as a negative control. Mean pixel density was quantified using CCD camera software analysis.

  • Human serum from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134003.

  • Human serum from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134003. Mean pixel density was quantified using CCD camera software analysis.

  • Left image: Conditioned media from iPSC-derived astrocytes; right image: Media only control.

    Samples were incubated overnight at 4C as recommended and included the large volume wash. Images were captured using CCD camera for the exposure times indicated on the image.

    Rating 5/5. Simple, sensitive and accurate method to detect multiple cytokines and growth factors from a single sample. The membranes were also consistent across the batch which allowed me to test several samples in parallel. Highly recommended.

Protocols

References

This product has been referenced in:
  • Cha JM  et al. Efficient scalable production of therapeutic microvesicles derived from human mesenchymal stem cells. Sci Rep 8:1171 (2018). Read more (PubMed: 29352188) »
  • Chen KY  et al. Arctigenin protects against steatosis in WRL68 hepatocytes through activation of phosphoinositide 3-kinase/protein kinase B and AMP-activated protein kinase pathways. Nutr Res 52:87-97 (2018). Read more (PubMed: 29525610) »
See all 2 Publications for this product

Customer reviews and Q&As

Question
Answer

For membrane-based arrays, any chemiluminescence imaging system such as an X-ray film developer, CCD camera, or gel documentation system should work. Imaging systems using near-infrared flours, such as the Li-Cor Odyssey and Typhoon systems, also work extremely well with our membrane arrays.

Data collection can be accomplished by any densitometry software. CCD camera-based imaging systems are typically equipped with a built-in densitometry software application. Alternatively, there is a free densitometry program available through the NIH (Image J), which can be downloaded here: http://rsb.info.nih.gov/ij/ More information on how to use Image J can be found here: http://imagejdocu.tudor.lu/imagej-documentation-wiki.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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