Overview

  • Product name

    Human Anti-Influenza virus A IgA ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Pos. Serum 8 2.1%
    Inter-assay
    Sample n Mean SD CV%
    Pos. Serum 19 3.7%
  • Sample type

    Serum, Hep Plasma, Cit plasma
  • Assay type

    Indirect
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s anti-Influenza virus A IgA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgA class antibodies against Influenza virus A in Human serum and plasma.


    A 96-well plate has been precoated with Influenza virus A antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgA conjugate is added to the wells, which binds to the immobilized Influenza virus A-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Influenza virus A IgA sample captured in plate.

  • Tested applications

    Suitable for: Indirect ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent dilution.

Protocols

References

ab108743 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your patience. Unfortunately, these kits have not been tested with the virus strains you are using and also not with nasal swaps and we do not know if this would work. I also have an additional concern: I understand you want to detect IgA antibodies which are synthesized as response to a vaccination with Influenza antigens. We have no data or literature about that but is very unlikely that the body will create IgA antibodies. This kind of antibody is a response of the mucosa to contact with a pathogen. In case of a vaccination there is no mucosa contact and hence I believe the production of IgA antibodies is improbable unless the vaccination is being administered intra nasally. I am sorry that I could not provide you with a positive answer on this occasion and hope this information is nevertheless helpful.

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Answer

Thank you for your inquiry. Ploease see the answer to your questions below: 1. For Influenza A IgA ELISA, ab108743, the wells are coated with purified Influenza A grade 2 antigens, strain Texas 1/77 (H3N2). For Influenza B IgA ELISA, ab108744, the wells are coated with purified Influenza B grade 2 antigens, strain Honk Kong 5/72. I can confirm that this ia a mixture of antigens and not only HA antigen. 2. I can confirm that the controls and cut-off control consists of positive human serum with preservative. Unfortunately, we do not sell human anti-influenza HA IgA serum at the moment. But I believe this is not necessary with this kit, since the control already consists of positive human serum. I hope this information is helpful and wish you good luck with your research.

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