Question (30559) | Human Anti-Influenza virus B IgA ELISA Kit (ab108744)

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Question

Dear Abcam, We are intrested in your 2 products, Influenza Virus A IgA Human ELISA Kit (ab108743) and Influenza Virus B IgA Human ELISA Kit (ab108744). However, we have two issues for you. 1. Can you tell us which flu antigen is coated on the strip? Whole influenza virus, splitted flu antigen or influenza HA protein only or other any form of flu antigen? 2. Can you tell me what immunogen are postive control and cut-off control serum against to, ex. whole flu virus, flu HA only or other epitope? We are intended to analyse influenza split vaccine immunized human serum and we concern about the vaccine might just contain HA antigen. By the way, do you sell human anti-influenza HA IgA serum which can be used for standard control and quantitation standard curve? Thanks for your time. Best regards,

Answer

Thank you for your inquiry. Ploease see the answer to your questions below: 1. For Influenza A IgA ELISA, ab108743, the wells are coated with purified Influenza A grade 2 antigens, strain Texas 1/77 (H3N2). For Influenza B IgA ELISA, ab108744, the wells are coated with purified Influenza B grade 2 antigens, strain Honk Kong 5/72. I can confirm that this ia a mixture of antigens and not only HA antigen. 2. I can confirm that the controls and cut-off control consists of positive human serum with preservative. Unfortunately, we do not sell human anti-influenza HA IgA serum at the moment. But I believe this is not necessary with this kit, since the control already consists of positive human serum. I hope this information is helpful and wish you good luck with your research.