Question (31556) | Human Anti-Influenza virus B IgA ELISA Kit (ab108744)

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Question

Dear Abcam, Thanks for your reply. We intend to monitor anit-flu serum IgA from people who are vaccined with t rivalent flu vaccine, which virus strains a re A/California/7/2009 (H1N1), A/Victoria/210/2009 (H3N2) and B/Brisbane/60/2008. Here are our further inquiry. 1. Are ab108743 and ab108744 suitable for detecting our serum sample? Since the coated antigen seems not exactly the same as our vaccine strain. 2. Could we detect IgA from nasal swab sample with your ELISA kit? Do you have suggested sample preparation method for nsasal swab sample ? Thanks for your time. Best regard,

Answer

Thank you for your patience. Unfortunately, these kits have not been tested with the virus strains you are using and also not with nasal swaps and we do not know if this would work. I also have an additional concern: I understand you want to detect IgA antibodies which are synthesized as response to a vaccination with Influenza antigens. We have no data or literature about that but is very unlikely that the body will create IgA antibodies. This kind of antibody is a response of the mucosa to contact with a pathogen. In case of a vaccination there is no mucosa contact and hence I believe the production of IgA antibodies is improbable unless the vaccination is being administered intra nasally. I am sorry that I could not provide you with a positive answer on this occasion and hope this information is nevertheless helpful.