Human Insulin degrading enzyme / IDE peptide (ab32215)

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Overview

Description

  • Nature
    Synthetic
  • Amino Acid Sequence
    • Species
      Human

Associated products

Specifications

Our Abpromise guarantee covers the use of ab32215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-Insulin degrading enzyme / IDE antibody (ab32216)

  • Form
    Liquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • Abeta-degrading protease
    • FLJ35968
    • Ide
    • IDE_HUMAN
    • Insulin protease
    • Insulin-degrading enzyme
    • Insulinase
    • Insulysin
    • OTTHUMP00000020097
    see all
  • Function
    Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia.
  • Sequence similarities
    Belongs to the peptidase M16 family.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localization
    Cytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.
  • Information by UniProt

References

ab32215 has not yet been referenced specifically in any publications.

Publishing research using ab32215? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1 Review or Q&A

10
Votes: Highest First

We wish to use IDE to check its enzyme activity with insulin as well as our peptide. Basically its catalytic activity is to be studied. Please let me know if we can use this product for our work or not.

This peptide (ab32215) can be used with studies using https://www.abcam.com/ab32216.html as blocking peptide to check any potencial non-specific signal. It has not been tested in any functional and activity assays.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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