Key features and details
- Sensitivity: 4 µlU/ml
- Range: 4.69 µlU/ml - 300 µlU/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman Insulin ELISA Kit
See all Insulin kits
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 4 µlU/ml
Range4.69 µlU/ml - 300 µlU/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 76.16 68% - 88% Serum 91.4 83% - 102% Plasma 99.03 73% - 128%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
Abcam’s Human Insulin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Insulin and Proinsulin in serum, plasma, cell culture supernatants.
This assay employs an antibody specific for Human Insulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Insulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Insulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Insulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This kit detects both insulin and proinsulin.
Get higher sensitivity in only 90 minutes with Human Insulin ELISA Kit (ab200011) from our SimpleStep ELISA® range.
Optimization may be required with urine samples.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human Insulin 2 vials Insulin Microplate (12 strips x 8 wells) 1 unit Recombinant Human Insulin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
FunctionInsulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Involvement in diseaseDefects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
Sequence similaritiesBelongs to the insulin family.
- Information by UniProt
Human Insulin measured in cell culture supernatants showing quantity (µIU) per mL of tested sample. Samples diluted 1-2 fold.
Human Insulin measured in biological fluids showing quantity (µIU) per mL of tested sample. Samples diluted 1-6 fold.
Human Insulin standard curve: mean of duplicates (+/- SD), performed in Diluent A
Representative Standard Curve using ab100578.
Representative Standard Curve using ab100578.
ab100578 has been referenced in 24 publications.
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- Fei H et al. MicroRNA-18 promotes apoptosis of islet ß-cells via targeting NAV1. Exp Ther Med 18:389-396 (2019). PubMed: 31258677
- Jiménez-Maldonado A et al. Moderate and high intensity chronic exercise reduces plasma tumor necrosis factor alpha and increases the Langerhans islet area in healthy rats. J Musculoskelet Neuronal Interact 19:354-361 (2019). PubMed: 31475943
- Citro A et al. Biofabrication of a vascularized islet organ for type 1 diabetes. Biomaterials 199:40-51 (2019). PubMed: 30735895
- Xu Y et al. Effects of citrate ester plasticizers and bis (2-ethylhexyl) phthalate in the OECD 28-day repeated-dose toxicity test (OECD TG 407). Environ Res 172:675-683 (2019). PubMed: 30878739