Human Insulin ELISA Kit (ab200011)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameHuman Insulin ELISA Kit
See all Insulin kits -
Detection methodColorimetric
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Precision
Intra-assay Sample n Mean SD CV% Overall 5 8.6% Inter-assay Sample n Mean SD CV% Overall 3 4.9% -
Sample typeSerum, Heparin Plasma
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Assay typeSandwich (quantitative)
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Sensitivity1.1 pmol/L
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Range2.1 pmol/L - 133.6 pmol/L
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Recovery
Sample specific recovery Sample type Average % Range Serum 106 103% - 111% Heparin Plasma 112 108% - 120% -
Assay time1h 30m
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Assay durationOne step assay
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Species reactivityReacts with: Human
Does not react with: Sheep, Goat, Guinea pig, Dog -
Product overview
Abcam’s Insulin in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Insulin protein in Human serum and plasma (heparin).
The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
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Notes
Insulin is a highly conserved, secreted hormone essential for glucose metabolism. Produced by pancreatic beta cells, proinsulin is proteolyzed into an A and a B chain, which form a 6 kDa mature protein. Basal levels of insulin are continuously delivered into the bloodstream, and additional levels are secreted proportional to food ingestion. Insulin secretion is highly regulated, and dysregulation of insulin production or sensitivity results in Type 1 diabetes mellitus or Type 2 diabetes mellitus, respectively.
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformMicroplate (12 x 8 well strips)
Properties
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Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests 10X Human Insulin Capture Antibody 1 x 600µl 10X Human Insulin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml ab221825 - Antibody Diluent 5BI 1 x 6ml Human Insulin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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FunctionInsulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
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Involvement in diseaseDefects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. -
Sequence similaritiesBelongs to the insulin family.
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Cellular localizationSecreted.
- Information by UniProt
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Alternative names
- IDDM2
- ILPR
- ins
see all -
Database links
- Entrez Gene: 3630 Human
- Omim: 176730 Human
- SwissProt: P01308 Human
- Unigene: 272259 Human
Associated products
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SimpleStep ELISA kits
Applications
Our Abpromise guarantee covers the use of ab200011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Example of Insulin standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Titration of Human serum and Human plasma within the working range of the assay.Data shown for serum is from an individual donor with high endogenous insulin levels. Data shown for plasma is from a pooled (n=50) sample. Background-subtracted data values (mean +/- SD, n = 3) are graphed.
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Insulin levels in individual healthy donors.Ten individual healthy donors were evaluated for the presence of Insulin in serum using this assay. The range was from 11.8 – 345.1 pmol/L, with an average of 99.4 pmol/L. Health history and dietary status of donors were unknown.
Protocols
References
This product has been referenced in:
- Zhao L et al. Chitosan oligosaccharide improves the therapeutic efficacy of sitagliptin for the therapy of Chinese elderly patients with type 2 diabetes mellitus. Ther Clin Risk Manag 13:739-750 (2017). Read more (PubMed: 28721055) »
