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    human-interferon-alpha-1-matched-antibody-pair-kit-ab215408.pdf

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Immunology Innate Immunity Cytokines Interferons
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Recombinant

Human Interferon alpha 1 Matched Antibody Pair Kit (ab215408)

  • Datasheet
  • SDS
  • Protocol Booklet
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Human Interferon alpha 1 standard curve.
  • Sandwich ELISA - Human Interferon alpha 1 Matched Antibody Pair Kit (ab215408)

Key features and details

  • Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
  • For economical ELISA and ELISA-based assay development
  • Reacts with: Human
  • Range: 2.34 pg/ml - 150 pg/ml

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Protein
Product image
Recombinant human Interferon alpha 1 protein (Active) (ab48750)

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Overview

  • Product name

    Human Interferon alpha 1 Matched Antibody Pair Kit
    See all Interferon alpha 1 kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Sensitivity

    0.64 pg/ml
  • Range

    2.34 pg/ml - 150 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human Interferon alpha 1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Interferon alpha 1.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab213479. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.

  • Tested applications

    Suitable for: IA, ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 5 x 96 tests
    Human Interferon alpha 1 Capture Antibody 2 x 50µg 1 x 50µg
    Human Interferon alpha 1 Detector Antibody 2 x 12.5µg 1 x 12.5µg
    Human Interferon alpha 1 Lyophilized Protein 2 vials 1 vial
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interferons
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interferons
  • Function

    Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
  • Sequence similarities

    Belongs to the alpha/beta interferon family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P01562 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • IFL
    • IFN
    • IFN alpha
    • IFN alpha 1/13
    • IFN alpha 1b
    • IFN alphaD
    • IFN-alpha-1/13
    • IFNA@
    • IFNA1
    • IFNA1_HUMAN
    • IFNA13
    • Interferon alpha 1
    • Interferon alpha-1/13
    • Interferon alpha-D
    • Interferon alpha1
    • Interferon, alpha 13
    • LeIF D
    see all
  • Database links

    • Entrez Gene: 3439 Human
    • Entrez Gene: 3447 Human
    • Omim: 147660 Human
    • SwissProt: P01562 Human
    • Unigene: 37026 Human
    • Unigene: 533471 Human

    Associated products

    • Alternative Versions

      • Human Interferon alpha 1 Antibody Pair - BSA and Azide free (ab241801)
    • Related Products

      • ELISA Accessory Pack (ab210905)
    • SimpleStep ELISA kits

      • Human Interferon alpha 1 ELISA Kit (ab213479)

    Applications

    Our Abpromise guarantee covers the use of ab215408 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IA Use at an assay dependent concentration.
    ELISA Use at an assay dependent concentration.

    Images

    • Human Interferon alpha 1 standard curve.
      Human Interferon alpha 1 standard curve.

      Standard calibration curve. Background subtracted values are graphed.

    • Sandwich ELISA - Human Interferon alpha 1 Matched Antibody Pair Kit (ab215408)
      Sandwich ELISA - Human Interferon alpha 1 Matched Antibody Pair Kit (ab215408)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab215408? Please let us know so that we can cite the reference in this datasheet.

    ab215408 has not yet been referenced specifically in any publications.

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