Human Interferon alpha 1 Matched Antibody Pair Kit (ab215408)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 2.34 pg/ml - 150 pg/ml
Overview
-
Product name
Human Interferon alpha 1 Matched Antibody Pair Kit
See all Interferon alpha 1 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
0.64 pg/ml -
Range
2.34 pg/ml - 150 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human Interferon alpha 1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Interferon alpha 1.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab213479. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
-
Tested applications
Suitable for: IA, ELISAmore details -
Platform
Reagents
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human Interferon alpha 1 Capture Antibody 2 x 50µg 1 x 50µg Human Interferon alpha 1 Detector Antibody 2 x 12.5µg 1 x 12.5µg Human Interferon alpha 1 Lyophilized Protein 2 vials 1 vial -
Research areas
-
Function
Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. -
Sequence similarities
Belongs to the alpha/beta interferon family. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- IFL
- IFN
- IFN alpha
see all -
Database links
- Entrez Gene: 3439 Human
- Entrez Gene: 3447 Human
- Omim: 147660 Human
- SwissProt: P01562 Human
- Unigene: 37026 Human
- Unigene: 533471 Human
Associated products
-
Alternative Versions
-
Related Products
-
SimpleStep ELISA kits
Applications
Our Abpromise guarantee covers the use of ab215408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IA | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. |
Images
-
Human Interferon alpha 1 standard curve.
Standard calibration curve. Background subtracted values are graphed.
-
Sandwich ELISA - Human Interferon alpha 1 Matched Antibody Pair Kit (ab215408)To learn more about the advantages of recombinant antibodies see here.
References (0)
ab215408 has not yet been referenced specifically in any publications.
