Overview

  • Product name

    Human Interferon Alpha 2 ELISA Kit
    See all Interferon alpha 2 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Spiked serum 3 2.7%
    Inter-assay
    Sample n Mean SD CV%
    Spiked serum 8 9.3%
  • Sample type

    Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    56.04 pg/ml
  • Range

    187.5 pg/ml - 12000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 103 99% - 109%
    Cell culture media 106 100% - 116%
    Heparin Plasma 104 94% - 108%
    EDTA Plasma 87 80% - 93%
    Citrate Plasma 103 102% - 103%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Interferon Alpha 2 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Interferon Alpha 2 protein in human sample 1, sample 2 and sample 3.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Interferon-alpha (IFN-alpha) 2 is produced by macrophages and is known to have antiviral activities. IFN-alpha 2 is a member of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells. Genetically, mouse and rat both share 62 percent sequence homology with human IFN-alpha 2.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BI 1 x 6ml
    10X Human Interferon Alpha 2 Capture Antibody 1 x 600µl
    10X Human Interferon Alpha 2 Detector Antibody 1 x 600µl
    Human Interferon Alpha 2 Lyophilized Purified Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Produced by macrophages, IFN-alpha have antiviral activities.
  • Sequence similarities

    Belongs to the alpha/beta interferon family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Alpha 2a interferon
    • IFN alpha 2
    • IFN alphaA
    • IFN-alpha-2
    • IFNA
    • Ifna2
    • IFNA2_HUMAN
    • IFNA2B
    • INFA2
    • Interferon
    • Interferon alpha 2a
    • Interferon alpha 2b
    • Interferon alpha A
    • Interferon alpha-2
    • Interferon alpha-A
    • LeIF A
    • LeIFA
    • MGC125764
    • MGC125765
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab233622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Interferon Alpha 2 were measured in duplicates, interpolated from the Interferon Alpha 2 standard curves.

Protocols

References

ab233622 has not yet been referenced specifically in any publications.

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