Human Interferon Gamma + IL2 ELISPOT Kit (ab48448)
Key features and details
- Assay type: Sandwich (qualitative)
- Detection method: Colorimetric
- Sample type: Suspension cells
- Reacts with: Human
Overview
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Product name
Human Interferon Gamma + IL2 ELISPOT Kit
See all Interferon Gamma + IL2 kits -
Detection method
Colorimetric -
Sample type
Suspension cells -
Assay type
Sandwich (qualitative) -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, oncology, infectious diseases, autoimmune diseases and transplantation.
This Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.
The dual colour Elispot allows you to monitor the production of two cytokines simultaneously in the same well.
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Tested applications
Suitable for: ELISpotmore details -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 5 x 96 tests 10 x 96 tests 10 x concentrate buffer for the preparation of AEC buffer 1 x 5ml 2 x 5ml 50 x concentrate AEC substrate buffer 1 x 1ml 2 x 1ml 96 PVDF-bottomed-well plates. 5 units 10 units Anti-FITC antibody HRP conjugate 1 x 120µl 2 x 100µl Bovine Serum Albumin 1 x 1g 2 x 1g Human IFN Gamma Capture antibody 1 x 500µl 2 x 500µl Human IL-2 Capture antibody 1 x 500µl 2 x 500µl IFNγ FITC conjugated detection antibody 1 vial 2 vials IL-2 Biotinylated detection antibody 1 vial 2 vials Ready-to-use BCIP/NBT substrate buffer 2 x 27ml 2 x 50ml Streptavidin - Alkaline Phosphatase conjugated 1 x 50µl 2 x 50µl -
Research areas
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Relevance
IFN-gamma is produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
Cellular localization
Secreted -
Alternative names
- IFN gamma
- IFNG
- IL2
see all -
Database links
- Entrez Gene: 3458 Human
- Entrez Gene: 3558 Human
- SwissProt: P01579 Human
- SwissProt: P60568 Human
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab48448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISpot |
Use at an assay dependent dilution.
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| Notes |
|---|
|
ELISpot
Use at an assay dependent dilution. |
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab48448 has not yet been referenced specifically in any publications.