Product nameHuman Interferon Gamma + IL2 FLUOROSPOT Kit
See all Interferon Gamma + IL2 kits
Sample typeCell culture supernatant, Cell culture extracts
Assay typeSandwich (qualitative)
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, oncology, infectious diseases, autoimmune diseases and transplantation.
This Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.
Tested applicationsSuitable for: FluroSpotmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 5 x 96 tests 10 x 96 tests 1 x 96 tests Anti-FITC antibody green fluorescence conjugate. 1 vial 1 vial 1 vial Biotinylated detection antibody for IL-2 (resuspend in 0.55mL). 1 vial 2 vials 1 vial Bovine Serum Albumin 1 x 2g 2 x 2g 1 x 2g FITC conjugated detection antibody for IFNg (resuspend in 0.55mL) 1 vial 2 vials 1 vial Human IFN Gamma Capture antibody 1 x 500µl 2 x 500µl 1 x 100µl Human IL-2 Capture antibody 1 x 500µl 2 x 500µl 1 x 100µl Streptavidin-phycoerythrin conjugate. 1 vial 1 vial 1 vial
RelevanceIFN-gamma is produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
- IFN gamma
Our Abpromise guarantee covers the use of ab48452 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|FluroSpot||Use at an assay dependent dilution.|
ab48452 has not yet been referenced specifically in any publications.