Overview

  • Product name

    Human Interferon gamma ELISA Kit (IFNG)
    See all Interferon gamma kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 15 pg/ml
  • Range

    20.6 pg/ml - 15000 pg/ml
  • Recovery

    > 87 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.53 84% - 104%
    Plasma 86.82 81% - 102%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Interferon gamma (IFNG) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Interferon gamma- in plasma, and cell culture supernatants. (Human Interferon gamma concentration is pretty low in normal plasma, it may not be detected in this assay).


    This assay employs an antibody specific for Human Interferon gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and Interferon gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Interferon gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Interferon gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect endogenous Human Interferon gamma in normal serum with ab100537, only in serum spiked with Human Interferon gamma.


    Get results in 90 minutes with Human IFN gamma ELISA Kit (ab174443) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human Interferon gamma (lyophilized) 2 vials
    Interferon gamma Microplate (12 x 8 wells) 1 unit
    Recombinant Human Interferon gamma Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Function

    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
  • Tissue specificity

    Released primarily from activated T lymphocytes.
  • Involvement in disease

    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
  • Sequence similarities

    Belongs to the type II (or gamma) interferon family.
  • Post-translational
    modifications

    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • IF 1
    • IFG
    • IFI
    • IFN gamma
    • IFN immune
    • IFN, immune
    • IFN-gamma
    • IFNG
    • IFNG_HUMAN
    • Immune interferon
    • Interferon gamma
    • Type II Interferon
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab100537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100537

  • Representative standard curve using ab100537

Protocols

References

ab100537 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your patience. I have checked on the bovine IFN-gamma ELISA Kit. Unfortunately, the kit currently is not available in our catalog as the quality control tests have not yet been completed.  In general, due to the low homology between the human and bovine as well as guinea pig proteins, a human IFN gamma ELISA kit might most likely not work well for these two species, very unfortunately. I also checked our IFN gamma antibodies that were tested in ELISA, but unfortunately, we do not have any that were tested in bovine or guinea pig either. I am sorry to have no better news for you at this moment. Please do not hesitate to contact us if you need any more advice or information. 

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Answer

Thank you for contacting us.   We have the following products in our catalog: ab108863: Interferon gamma Human ELISA Kit We have tested the cross-reactivity with bovine samples and it is very low, around 1 %. We have never tested samples from guinea pig. ab100537: Interferon gamma Human ELISA Kit - 1 x 96 Well Plate ab100689: Interferon gamma Mouse ELISA Kit - 1 x 96 Well Plate Guinea pig IFN-gamma shares ~56% and ~41% to human and mouse respectively. Therefore this protein doesn’t seem to be that conserved between species and as these ELISA kits are sandwich-based and very species specific, I would expect minimal to zero cross reactivity with a guinea pig sample. The only way to know for sure would be to test and see. However, we might be able to add a bovine IFN-gamma ELISA Kit to our catalog. I will check and let you know. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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