Overview

  • Product name
    Human Interferon gamma ELISA Kit (IFNG)
    See all Interferon gamma kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 5.2%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.8%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    > 0.01 ng/ml
  • Range
    0.016 ng/ml - 1 ng/ml
  • Recovery

    101 %

  • Assay time
    5h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Interferon gamma (IFNG) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Interferon gamma levels in cell culture supernatants, serum and plasma.

    An Interferon gamma specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Interferon gamma specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Interferon gamma captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Images

  • Representative Standard Curve using ab108863.

Protocols

References

ab108863 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your patience. I have checked on the bovine IFN-gamma ELISA Kit. Unfortunately, the kit currently is not available in our catalog as the quality control tests have not yet been completed.  In general, due to the low homology between the human and bovine as well as guinea pig proteins, a human IFN gamma ELISA kit might most likely not work well for these two species, very unfortunately. I also checked our IFN gamma antibodies that were tested in ELISA, but unfortunately, we do not have any that were tested in bovine or guinea pig either. I am sorry to have no better news for you at this moment. Please do not hesitate to contact us if you need any more advice or information. 

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Answer

Thank you for contacting us.   We have the following products in our catalog: ab108863: Interferon gamma Human ELISA Kit We have tested the cross-reactivity with bovine samples and it is very low, around 1 %. We have never tested samples from guinea pig. ab100537: Interferon gamma Human ELISA Kit - 1 x 96 Well Plate ab100689: Interferon gamma Mouse ELISA Kit - 1 x 96 Well Plate Guinea pig IFN-gamma shares ~56% and ~41% to human and mouse respectively. Therefore this protein doesn’t seem to be that conserved between species and as these ELISA kits are sandwich-based and very species specific, I would expect minimal to zero cross reactivity with a guinea pig sample. The only way to know for sure would be to test and see. However, we might be able to add a bovine IFN-gamma ELISA Kit to our catalog. I will check and let you know. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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