Human Interferon gamma ELISPOT Kit (Reagents for 10 x 96 tests, without plates) (ab46604)

Submit an Abreview Submit a Question

Overview

  • Product name
    Human Interferon gamma ELISPOT Kit (Reagents for 10 x 96 tests, without plates)
    See all Interferon gamma kits
  • Sample type
    Cell culture supernatant, Cell culture extracts
  • Assay type
    Sandwich (qualitative)
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Intended use

    This ELISPOT assay is designed to measure cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. This Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, oncology, infectious diseases, autoimmune diseases and transplantation.

    The Interferon gamma Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

    Principle of the method

    After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.

     

    Recognizes natural human Interferon gamma

  • Tested applications
    Suitable for: ELISpotmore details
  • Platform
    Reagents

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 10 x 96 tests
    Biotinylated Human IFNγ Detection Antibody 2 vials
    Human IFN Gamma  Capture antibody 2 x 0.5ml
  • Research areas
  • Relevance
    Interferon gamma is a dimerized soluble cytokine that is the only member of the type II class of interferons. In contrast to interferon alpha and interferon beta which can be expressed by all cells, Interferon gamma is secreted by T lymphocytes and NK cells only. Also known as immune interferon, Interferon gamma is the only Type II interferon. It is serologically distinct from Type I interferons and it is acid-labile, while the type I variants are acid-stable. Interferon gamma has antiviral, immunoregulatory, and anti-tumour properties. It alters transcription in up to 30 genes producing a variety of physiological and cellular responses. Activation by Interferon gamma is achieved by its interaction with a heterodimeric receptor consisting of IFNGR1 & IFNGR2 (interferon gamma receptors). Interferon gamma binding to the receptor activates the JAK-STAT pathway. In addition, Interferon gamma activates APCs and promotes Th1 differentiation by upregulating the transcription factor T-bet. Interferon gamma is the hallmark cytokine of Th1 cells (Th2 cells produce IL-4). NK cells and CD8+ cytotoxic T cells also produce Interferon gamma. Interferon gamma suppresses osteoclast formation by rapidly degrading the RANK adaptor protein TRAF6 in the RANK-RANKL signaling pathway, which otherwise stimulates the production of NF?B.
  • Cellular localization
    Secreted
  • Alternative names
    • IFG
    • IFI
    • IFN gamma
    • IFNG
    • Immune interferon
    see all
  • Database links

    Applications

    Our Abpromise guarantee covers the use of ab46604 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISpot
  • Application notes
    ELISPOT: Please refer to specific protocol.

    Optimal dilutions/concentrations should be determined by the end user.

    • References

    ab46604 has not yet been referenced specifically in any publications.

    Publishing research using ab46604? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab46604.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up