Overview

  • Product name

    Human Interferon gamma Receptor 1 ELISA Kit (CD119)
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    MCF7 Lysate 9 4.9%
    Inter-assay
    Sample n Mean SD CV%
    MCF7 Lysate 3 6.2%
  • Sample type

    Cell culture extracts, Adherent cells, Suspension cells, Cell Lysate, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    140 pg/ml
  • Range

    0.14 ng/ml - 100 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 82 72% - 87%
    Plasma 78 73% - 85%
    Cell culture media 84 80% - 94%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Interferon-gamma Receptor 1 (CD119) human in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Interferon-gamma Receptor 1 protein in cell lysates and tissue extracts.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Interferon gamma receptor 1 (IFNGR1) is a heterodimeric receptor that binds one interferon gamma dimer as a ligand. The receptor is a single-pass type I membrane protein present on the plasma membrane. A genetic variation in the IFNGR1 gene is associated with susceptibility to Helicobacter pylori infection. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD); also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab173193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example IFNGR1 standard curve. Background-subtracted data values (mean +/- SD) are graphed. A new standard curve must be generated for each assay performed.

  • Titration of MCF7 cell lysate within the working range of the assay. Background subtracted data from triplicate measurements are plotted.

  • Quantification of IFNGR1 expression in different mammalian cell lines. Interpolated values of IFNGR1 are plotted for the indicated cell lines based on a lysate concentration of 100 µg/mL.

Protocols

References

ab173193 has not yet been referenced specifically in any publications.

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