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|Sample type||Average %||Range|
|Serum||82||72% - 87%|
|Plasma||78||73% - 85%|
|Cell culture media||84||80% - 94%|
Interferon-gamma Receptor 1 (CD119) human in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Interferon-gamma Receptor 1 protein in cell lysates and tissue extracts.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Interferon gamma receptor 1 (IFNGR1) is a heterodimeric receptor that binds one interferon gamma dimer as a ligand. The receptor is a single-pass type I membrane protein present on the plasma membrane. A genetic variation in the IFNGR1 gene is associated with susceptibility to Helicobacter pylori infection. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD); also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis.
|Components||1 x 96 tests|
|10X IFNGR1 Capture Antibody||1 x 600µl|
|10X IFNGR1 Detector Antibody||1 x 600µl|
|10X Wash Buffer PT (ab206977)||1 x 20ml|
|50X Cell Extraction Enhancer Solution (ab193971)||1 x 1ml|
|5X Cell Extraction Buffer PTR (ab193970)||1 x 10ml|
|Antibody Diluent 5B||1 x 6ml|
|IFNGR1 Human Lyophilized Recombinant Protein||2 x 50ng|
|Plate Seals||1 unit|
|Sample Diluent NS||1 x 12ml|
|SimpleStep Pre-Coated 96-Well Microplate (ab206978)||1 unit|
|Stop Solution||1 x 12ml|
|TMB Development Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab173193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Example IFNGR1 standard curve. Background-subtracted data values (mean +/- SD) are graphed. A new standard curve must be generated for each assay performed.
Titration of MCF7 cell lysate within the working range of the assay. Background subtracted data from triplicate measurements are plotted.
Quantification of IFNGR1 expression in different mammalian cell lines. Interpolated values of IFNGR1 are plotted for the indicated cell lines based on a lysate concentration of 100 µg/mL.
ab173193 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"